Abstract
Cerium-based methods have been used for the demonstration of seve phatases at alkaline, acid and neutral pH in low temperature acetone-fixed, plastic-e sections. At alkaline pH calcium is used as capturing agent and the precipitated phosphate converted to cerium phosphate. At neutral and acid pH cerium is used d capturing agent. Cerium phosphate is subsequently visualized using the H2C2DAB n: comparison has been made with conventional calcium-cobalt and lead methods. It; that calcium-cobalt methods are more susceptible to improvement than lead methods
Original language | English |
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Pages (from-to) | 289-296 |
Number of pages | 8 |
Journal | Stain technology |
Volume | 64 |
Issue number | 6 |
DOIs | |
Publication status | Published - Nov-1989 |