Changes in T and B cell subsets in end stage renal disease patients before and after kidney transplantation

Lei Wang, Christien Rondaan, Anoek A. E. de Joode, Elisabeth Raveling-Eelsing, Nicolaas A. Bos, Johanna Westra*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Background The incidence of kidney transplantation performed in elderly patients has increased steadily recently. Higher risk of infection and mortality, but lower rate of rejection, are reported in older kidney transplant patients. This study aims to analyze the effect of transplantation on aging of T and B cells in kidney transplant patients, with the emphasis on age and Cytomegalovirus (CMV) latency. Results We included 36 patients before and after (median 2.7 years) kidney transplantation and 27 age- and sex-matched healthy controls (HC). T and B cell subsets were measured by flow cytometry, with a focus on aged T cells (CD28-), and age associated B cells (ABCs, CD19 + CD21-CD11c+). Three years after transplantation a significant increase of total T cells among the lymphocytes was found compared to pre-transplantation and HC. Among the T cells CD4+ cells were decreased, especially naive CD4+ cells and regulatory T cells. Total CD8+ cell proportions were increased, and proportions of naive CD8+ cells were significantly decreased after transplantation, while CD8+ effector memory T cells re-expressing CD45RA were increased. CD28- T cells were significantly higher compared to HC after transplantation, especially in CMV seropositive patients. B cells were significantly decreased, while among B cells memory B cells and especially ABCs were increased after transplantation. Conclusions After transplantation T and B cell subsets change towards more terminally differentiated memory cells compared to age-matched HC. Proportions of aged T cells and ABCs were associated with CMV serostatus.

Original languageEnglish
Article number43
Number of pages11
JournalImmunity & ageing : I & A
Issue number1
Publication statusPublished - 8-Nov-2021


  • Kidney transplant
  • Ageing
  • CMV
  • Senescent T cells
  • Age associated B cells
  • AGE

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