Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel

Rik T. C. Cleophas; Jelmer Sjollema; Henk J. Busscher; John A. W. Kruijtzer; Rob M. J. Liskamp

doi:10.1021/bm500899r

Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel

Rik T. C. Cleophas, Jelmer Sjollema, Henk J. Busscher, John A. W. Kruijtzer, Rob M. J. Liskamp^*

^*Corresponding author for this work

Man, Biomaterials and Microbes (MBM)

Research output: Contribution to journal › Article › Academic › peer-review

52 Citations (Scopus)

Abstract

A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into a soft hydrogel network on a poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal hydrogel was molecularly characterized via Coomassie and Lowry assay protein staining agents as well as by X-ray photoelectron spectroscopy. The bactericidal activity was established against Staphylococcus aureus and Staphylococcus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain further insight into the biological stability, the hydrogels were incubated with human serum prior to activity testing without loss of activity. These studies revealed a promising bactericidal hydrogel with good stability under physiological conditions.

Original language	English
Pages (from-to)	3390-3395
Number of pages	6
Journal	Biomacromolecules
Volume	15
Issue number	9
DOIs	https://doi.org/10.1021/bm500899r
Publication status	Published - Sept-2014

Keywords

CATIONIC-PEPTIDE
BROAD-SPECTRUM
INFECTIONS
PREVENTION
ANTIBIOTICS
CHEMISTRY
IMPLANTS
SURFACES
COATINGS
TITANIUM

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Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel
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title = "Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel",

abstract = "A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into a soft hydrogel network on a poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal hydrogel was molecularly characterized via Coomassie and Lowry assay protein staining agents as well as by X-ray photoelectron spectroscopy. The bactericidal activity was established against Staphylococcus aureus and Staphylococcus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain further insight into the biological stability, the hydrogels were incubated with human serum prior to activity testing without loss of activity. These studies revealed a promising bactericidal hydrogel with good stability under physiological conditions.",

keywords = "CATIONIC-PEPTIDE, BROAD-SPECTRUM, INFECTIONS, PREVENTION, ANTIBIOTICS, CHEMISTRY, IMPLANTS, SURFACES, COATINGS, TITANIUM",

author = "Cleophas, {Rik T. C.} and Jelmer Sjollema and Busscher, {Henk J.} and Kruijtzer, {John A. W.} and Liskamp, {Rob M. J.}",

year = "2014",

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doi = "10.1021/bm500899r",

language = "English",

volume = "15",

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T1 - Characterization and Activity of an Immobilized Antimicrobial Peptide Containing Bactericidal PEG-Hydrogel

AU - Cleophas, Rik T. C.

AU - Sjollema, Jelmer

AU - Busscher, Henk J.

AU - Kruijtzer, John A. W.

AU - Liskamp, Rob M. J.

PY - 2014/9

Y1 - 2014/9

N2 - A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into a soft hydrogel network on a poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal hydrogel was molecularly characterized via Coomassie and Lowry assay protein staining agents as well as by X-ray photoelectron spectroscopy. The bactericidal activity was established against Staphylococcus aureus and Staphylococcus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain further insight into the biological stability, the hydrogels were incubated with human serum prior to activity testing without loss of activity. These studies revealed a promising bactericidal hydrogel with good stability under physiological conditions.

AB - A single step immobilization-polymerization strategy of a highly active antimicrobial peptide into a soft hydrogel network on a poly(ethylene terephthalate) surface using thiol-ene chemistry is described. The bactericidal hydrogel was molecularly characterized via Coomassie and Lowry assay protein staining agents as well as by X-ray photoelectron spectroscopy. The bactericidal activity was established against Staphylococcus aureus and Staphylococcus epidermidis, two bacterial strains commonly associated with biomaterial infections. To gain further insight into the biological stability, the hydrogels were incubated with human serum prior to activity testing without loss of activity. These studies revealed a promising bactericidal hydrogel with good stability under physiological conditions.

KW - CATIONIC-PEPTIDE

KW - BROAD-SPECTRUM

KW - INFECTIONS

KW - PREVENTION

KW - ANTIBIOTICS

KW - CHEMISTRY

KW - IMPLANTS

KW - SURFACES

KW - COATINGS

KW - TITANIUM

U2 - 10.1021/bm500899r

DO - 10.1021/bm500899r

M3 - Article

SN - 1525-7797

VL - 15

SP - 3390

EP - 3395

JO - Biomacromolecules

JF - Biomacromolecules

IS - 9

ER -