Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)

M Chevolot; TBH Reusch; S Boele-Bos; WT Stam; JL Olsen

doi:10.1111/j.1471-8286.2005.00951.x

Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)

M Chevolot^*, TBH Reusch, S Boele-Bos, WT Stam, JL Olsen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The thornback ray, Raja clavata, is an elasmobranch (cartilaginous fish). Since the 1950s, its stock has severely declined. In order to investigate the genetic population structure of the species, we developed microsatellite loci. The five loci reported here have eight to 48 alleles per locus and display an observed heterozygosity ranging from 0.32 to 0.98 with no deviation from the Hardy-Weinberg equilibrium. In the test panel of 122 individuals from three populations, no null alleles, band-stuttering, large allele dropouts or linkage disequilibrium was detected.

Original language	English
Pages (from-to)	427-429
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00951.x
Publication status	Published - Jun-2005

Keywords

elasmobranchs
microsatellites
Raja clavata
Rajidae
NORTH-SEA
SKATES

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Characterization and isolation of DNA microsatellite primers in Raja clavata
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title = "Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)",

abstract = "The thornback ray, Raja clavata, is an elasmobranch (cartilaginous fish). Since the 1950s, its stock has severely declined. In order to investigate the genetic population structure of the species, we developed microsatellite loci. The five loci reported here have eight to 48 alleles per locus and display an observed heterozygosity ranging from 0.32 to 0.98 with no deviation from the Hardy-Weinberg equilibrium. In the test panel of 122 individuals from three populations, no null alleles, band-stuttering, large allele dropouts or linkage disequilibrium was detected.",

keywords = "elasmobranchs, microsatellites, Raja clavata, Rajidae, NORTH-SEA, SKATES",

author = "M Chevolot and TBH Reusch and S Boele-Bos and WT Stam and JL Olsen",

year = "2005",

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doi = "10.1111/j.1471-8286.2005.00951.x",

language = "English",

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journal = "Molecular Ecology Notes",

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T1 - Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)

AU - Chevolot, M

AU - Reusch, TBH

AU - Boele-Bos, S

AU - Stam, WT

AU - Olsen, JL

PY - 2005/6

Y1 - 2005/6

N2 - The thornback ray, Raja clavata, is an elasmobranch (cartilaginous fish). Since the 1950s, its stock has severely declined. In order to investigate the genetic population structure of the species, we developed microsatellite loci. The five loci reported here have eight to 48 alleles per locus and display an observed heterozygosity ranging from 0.32 to 0.98 with no deviation from the Hardy-Weinberg equilibrium. In the test panel of 122 individuals from three populations, no null alleles, band-stuttering, large allele dropouts or linkage disequilibrium was detected.

AB - The thornback ray, Raja clavata, is an elasmobranch (cartilaginous fish). Since the 1950s, its stock has severely declined. In order to investigate the genetic population structure of the species, we developed microsatellite loci. The five loci reported here have eight to 48 alleles per locus and display an observed heterozygosity ranging from 0.32 to 0.98 with no deviation from the Hardy-Weinberg equilibrium. In the test panel of 122 individuals from three populations, no null alleles, band-stuttering, large allele dropouts or linkage disequilibrium was detected.

KW - elasmobranchs

KW - microsatellites

KW - Raja clavata

KW - Rajidae

KW - NORTH-SEA

KW - SKATES

U2 - 10.1111/j.1471-8286.2005.00951.x

DO - 10.1111/j.1471-8286.2005.00951.x

M3 - Article

SN - 1471-8278

VL - 5

SP - 427

EP - 429

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)

Abstract

Keywords

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