Characterization of human faecal flora by means of an improved fluoro-morphometrical method

G.J. Jansen; M.H.F. Wilkinson; B. Deddens; D.  van der Waaij

doi:10.1017/S0950268800056971

Characterization of human faecal flora by means of an improved fluoro-morphometrical method

G.J. Jansen, M.H.F. Wilkinson, B. Deddens, D. van der Waaij

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Abstract

The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria ('morphotypes') was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.

Original language	English
Pages (from-to)	265-272
Number of pages	8
Journal	Epidemiology And Infection
Volume	111
Issue number	2
DOIs	https://doi.org/10.1017/S0950268800056971
Publication status	Published - Oct-1993

Keywords

FECAL FLORA
ANTIBIOTICS
CEFTRIAXONE
MICROFLORA

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title = "Characterization of human faecal flora by means of an improved fluoro-morphometrical method",

abstract = "The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria ('morphotypes') was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.",

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author = "G.J. Jansen and M.H.F. Wilkinson and B. Deddens and {van der Waaij}, D.",

note = "Relation: http://www.rug.nl/informatica/organisatie/overorganisatie/iwi Rights: University of Groningen. Research Institute for Mathematics and Computing Science (IWI)",

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AU - Deddens, B.

AU - van der Waaij, D.

N1 - Relation: http://www.rug.nl/informatica/organisatie/overorganisatie/iwi Rights: University of Groningen. Research Institute for Mathematics and Computing Science (IWI)

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AB - The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria ('morphotypes') was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.

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KW - ANTIBIOTICS

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KW - MICROFLORA

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DO - 10.1017/S0950268800056971

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JO - Epidemiology And Infection

JF - Epidemiology And Infection

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ER -

Characterization of human faecal flora by means of an improved fluoro-morphometrical method

Abstract

Keywords

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