Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels

Patty Mulder; Sander Koster; Heiko J. Van Der Linden; Grietje Molema; Elisabeth Verpoorte

Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels

Patty Mulder^*, Sander Koster, Heiko J. Van Der Linden, Grietje Molema, Elisabeth Verpoorte

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

6 Citations (Scopus)

Abstract

Our long-term interest is to explore the potential of microfluidics for the development of advanced tools for cell studies and analysis. This contribution describes a method to cultivate Human Umbilical Vein Endothelial Cells (HUVEC) in microchannels without continuous flow. The effects of seeding and culture conditions on cell behavior were studied.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	1383-1385
Number of pages	3
ISBN (Print)	0974361119, 9780974361116
Publication status	Published - 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: 9-Oct-2005 → 13-Oct-2005

Publication series

Name	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume	1

Conference

Conference	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/Territory	United States
City	Boston, MA
Period	09/10/2005 → 13/10/2005

Keywords

Cell microcultivation
Cell morphology
Endothelial cells
PDMS

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Mulder, P., Koster, S., Van Der Linden, H. J., Molema, G., & Verpoorte, E. (2005). Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1383-1385). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.

Mulder, Patty ; Koster, Sander ; Van Der Linden, Heiko J. et al. / Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. pp. 1383-1385 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{0b22c7373e0e444ab9fdd94682eb33e6,

title = "Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels",

abstract = "Our long-term interest is to explore the potential of microfluidics for the development of advanced tools for cell studies and analysis. This contribution describes a method to cultivate Human Umbilical Vein Endothelial Cells (HUVEC) in microchannels without continuous flow. The effects of seeding and culture conditions on cell behavior were studied.",

keywords = "Cell microcultivation, Cell morphology, Endothelial cells, PDMS",

author = "Patty Mulder and Sander Koster and {Van Der Linden}, {Heiko J.} and Grietje Molema and Elisabeth Verpoorte",

year = "2005",

language = "English",

isbn = "0974361119",

series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Transducer Research Foundation",

pages = "1383--1385",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",

note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",

}

Mulder, P, Koster, S, Van Der Linden, HJ, Molema, G & Verpoorte, E 2005, Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, vol. 1, Transducer Research Foundation, pp. 1383-1385, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 09/10/2005.

Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels. / Mulder, Patty; Koster, Sander; Van Der Linden, Heiko J. et al.
Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. p. 1383-1385 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels

AU - Mulder, Patty

AU - Koster, Sander

AU - Van Der Linden, Heiko J.

AU - Molema, Grietje

AU - Verpoorte, Elisabeth

PY - 2005

Y1 - 2005

N2 - Our long-term interest is to explore the potential of microfluidics for the development of advanced tools for cell studies and analysis. This contribution describes a method to cultivate Human Umbilical Vein Endothelial Cells (HUVEC) in microchannels without continuous flow. The effects of seeding and culture conditions on cell behavior were studied.

AB - Our long-term interest is to explore the potential of microfluidics for the development of advanced tools for cell studies and analysis. This contribution describes a method to cultivate Human Umbilical Vein Endothelial Cells (HUVEC) in microchannels without continuous flow. The effects of seeding and culture conditions on cell behavior were studied.

KW - Cell microcultivation

KW - Cell morphology

KW - Endothelial cells

KW - PDMS

M3 - Conference contribution

AN - SCOPUS:84884343585

SN - 0974361119

SN - 9780974361116

T3 - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1383

EP - 1385

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference

PB - Transducer Research Foundation

T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005

Y2 - 9 October 2005 through 13 October 2005

ER -

Mulder P, Koster S, Van Der Linden HJ, Molema G , Verpoorte E. Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation. 2005. p. 1383-1385. (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Characterization of human umbilical vein endothelial cells (HUVEC) cultivated in microfluidic channels

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