Characterization of the CD8(+) T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice

Michael V. Lukens, Erwin A. W. Claassen, Patricia M. A. de Graaff, Maniska E. A. van Dijk, Peter Hoogerhout, Mireille Toebes, Ton N. Schumacher, Robbert G. van der Most, Jan L. L. Kimpen, Grada M. van Bleek*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

46 Citations (Scopus)

Abstract

The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4(+) and CD8(+). T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8+ T cells during primary and secondary responses in lung parenchyma,. airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8(+) T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice. (c) 2006 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalVirology
Volume352
Issue number1
DOIs
Publication statusPublished - 15-Aug-2006
Externally publishedYes

Keywords

  • respiratory syncytial virus
  • C57BL/6
  • CD8(+) T cells
  • inactivation
  • tetramer
  • BALB/C MICE
  • ANTIGEN PRESENTATION
  • INTERFERON-ALPHA
  • FUSION PROTEIN
  • INBRED MICE
  • IDENTIFICATION
  • VACCINE
  • TRACT
  • SUSCEPTIBILITY
  • EOSINOPHILIA

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