Characterization of urinary CD4⁺ and CD8⁺ T cells in kidney transplantation patients with polyomavirus BK infection and allograft rejection

W B van Doesum, W H Abdulahad, M. C. R. F. van Dijk, S Dolff, W J van Son, C A Stegeman, Jan Stephan Sanders

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2 Citations (Scopus)

Abstract

BACKGROUND AND OBJECTIVES: The objective of this study was to characterize CD4(+) and CD8(+) T-cell populations in blood and urine of renal transplant patients with BK virus (BKV) infection or allograft rejection.

MATERIALS AND METHODS: Percentages and absolute numbers of CD4(+) and CD8(+) effector memory T-cell subtype (TEM ) and terminal differentiated T cells (TTD ) in renal transplant patients with BKV infection (n = 14), with an episode of allograft rejection (n = 9), and in uncomplicated renal transplant patients with a stable kidney function (n = 12) were measured and compared using 4-color fluorescence-activated cell sorting. Results were correlated with the number of CD4(+) and CD8(+) T cells in renal biopsies.

RESULTS: In patients with allograft rejection, the number of urinary CD4(+) TEM and CD8(+) TEM cells was significantly increased compared to patients with BKV infection or patients without complications. Positive correlation was found between the number of CD4(+) and CD8(+) cells in the renal biopsies and the number of CD4(+) and CD8(+) cells in urine. In patients with rejection, after 2 months of immunosuppressive therapy, a reduction in urinary CD8(+) TEM cells was found.

CONCLUSIONS: CD4(+) TEM and CD8(+) TEM cells in urine could be a marker to distinguish allograft rejection from BKV-associated nephropathy and to monitor therapy effectiveness in renal transplant patients with allograft rejection.

Original languageEnglish
Pages (from-to)733-743
Number of pages11
JournalTransplant Infectious Disease
Volume16
Issue number5
DOIs
Publication statusPublished - 2014

Keywords

  • Adult
  • Aged
  • Allografts
  • BK Virus
  • Biopsy
  • CD4 Lymphocyte Count
  • CD4-Positive T-Lymphocytes
  • CD8-Positive T-Lymphocytes
  • Female
  • Graft Rejection
  • Humans
  • Immunosuppressive Agents
  • Kidney
  • Kidney Transplantation
  • Male
  • Middle Aged
  • Polyomavirus Infections
  • T-Lymphocyte Subsets
  • Tumor Virus Infections
  • Urine
  • Young Adult

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