Abstract
This review article underlines the importance of gas chromatography-mass spectrometry (GC-MS) for determination of steroids in man. The use of steroids labelled with stable isotopes as internal standard and subsequent analysis by GC-MS yields up to now the only reliable measurement of steroids in serum. Isotope dilution GC-MS is the reference method for evaluation of routine analysis of serum steroid hormones. GC-MS is an important tool for detection of steroid hormone doping and combined with a combustion furnace and an isotope ratio mass spectrometer the misuse of testosterone by athletes can be discovered. Finally the so called urinary steroid profile by GC and GC-MS is the method of choice for detection of steroid metabolites in health and disease. (C) 1999 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 247-274 |
| Number of pages | 28 |
| Journal | Journal of Chromatography A |
| Volume | 843 |
| Issue number | 1-2 |
| Publication status | Published - 28-May-1999 |
Keywords
- reviews
- steroids
- hormones
- CONGENITAL ADRENAL-HYPERPLASIA
- EXTERNAL QUALITY ASSESSMENT
- APPARENT MINERALOCORTICOID EXCESS
- GLUCOCORTICOID-REMEDIABLE ALDOSTERONISM
- CANDIDATE DEFINITIVE METHOD
- REFERENCE METHOD VALUES
- HUMAN-SERUM
- 21-HYDROXYLASE DEFICIENCY
- URINARY TESTOSTERONE
- REFERENCE LABORATORIES