Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-2(2) from Lycopersicon esculentum

FC Lanfermeijer*, J Dijkhuis, MJG Sturre, P de Haan, J Hille

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In tomato, infections by tomato mosaic virus are controlled by durable Tm-2(2) resistance. In order to gain insight into the processes underlying disease resistance and its durability, we cloned and analysed the Tm-2(2) resistance gene and the susceptible allele, tm-2. The Tm-2(2) gene was isolated by transposon tagging using a screen in which plants with a destroyed Tm-2(2) gene survive. The Tm-2(2) locus consists of a single gene that encodes an 861 amino acid polypeptide, which belongs to the CC-NBS-LRR class of resistance proteins. The putative tm-2 allele was cloned from susceptible tomato lines via PCR with primers based on the Tm-2(2) sequence. Interestingly, the tm-2 gene has an open reading frame that is comparable to the Tm-2(2) allele. Between the tm-2 and the Tm-2(2) polypeptide 38 amino acid differences are present of which 26 are located in the second half of the LRR-domain. Susceptible tomato plants, which were transformed with the Tm-2(2) gene, displayed resistance against ToMV infection. In addition, virus specificity, displayed by the Tm-2(2) resistance was conserved in these transgenic lines. To explain the durability of this resistance, it is proposed that the Tm-2(2)-encoded resistance is aimed at the Achilles' heel of the virus.

Original languageEnglish
Pages (from-to)1037-1049
Number of pages13
JournalPlant Molecular Biology
Volume52
Issue number5
Publication statusPublished - Jul-2003

Keywords

  • CC-NBS-LRR
  • Lycopersicon esculentum
  • plant disease resistance gene
  • Tm-2(2)
  • tomato mosaic virus
  • transposon tagging
  • RICH REPEAT CLASS
  • DISEASE RESISTANCE
  • CLADOSPORIUM-FULVUM
  • NUCLEOTIDE-BINDING
  • MOVEMENT PROTEIN
  • TOBACCO
  • LOCUS
  • PLANTS
  • MEMBER
  • ACID

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