Coculture of rat embryonic proprioceptive sensory neurons and myotubes

Sjef Copray*, Robert Liem, Ietje Mantingh-Otter, Nieske Brouwer

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    6 Citations (Scopus)

    Abstract

    With the aim to study the cellular mechanisms underlying the process of muscle spindle (re)generation, dorsal root ganglia (DRG) neurons derived from 16-day rat embryos were cocultured with developing myotubes in a compartmentalized culture device. To accomplish the selective survival and neurite formation of the proprioceptive subpopulation, the neurotrophic factor, neurotrophin-3, was added to the culture medium. It appeared that the proprioceptive DRG neurons could develop specialized, la afferent terminallike contacts with myotubes. However, these interactions were scarce and did not result in the induction of differentiation of the contacted myotubes into intrafusal fibers as normally occurs during in vivo development. The present coculture setup apparently lacks appropriate regulatory factors essential for the proper matching of sensory axons and intrafusal fiber precursors and the induction of a functional sensory myoneural connection. (C) 1996 John Wiley & Sons, Inc.

    Original languageEnglish
    Pages (from-to)1401-1412
    Number of pages12
    JournalMUSCLE & NERVE
    Volume19
    Issue number11
    DOIs
    Publication statusPublished - Nov-1996

    Keywords

    • proprioceptive sensory neurons
    • neurotrophin-3
    • myotubes
    • muscle spindles
    • intrafusal fibers
    • rat
    • NERVE GROWTH-FACTOR
    • INTRAFUSAL MUSCLE-FIBERS
    • MYOSIN HEAVY-CHAIN
    • NEUROMUSCULAR-JUNCTION
    • SKELETAL-MUSCLE
    • NEONATAL RATS
    • EXPRESSION
    • SPINDLES
    • NEUROTROPHIN-3
    • AFFERENTS

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