Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats: A macroscopic and morphological study

P M Werker; M Kon; C J Green; P R Fryer; R J Franken; M L Overgoor

doi:10.1002/micr.1920150309

Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats: A macroscopic and morphological study

P M Werker, M Kon, C J Green, P R Fryer, R J Franken, M L Overgoor

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The purpose of this study was to define the cold (0 degree C) ischaemic tolerance (CIT) of the latissimus dorsi free flap in inbred rats. Flaps were isolated, stored at 0 degree C for periods of time ranging from 0 to 120 hr and then revascularised and reperfused for 14 days. Thereafter the CIT was assessed macroscopically, and by light microscopy. Electron microscopy was performed in flaps after 0 and 24 hr of reperfusion following a cold ischaemic interval ranging from 0 to 120 hr. From this preliminary study, it was concluded that the CIT was 48-72 hr.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	Microsurgery
Volume	15
Issue number	3
DOIs	https://doi.org/10.1002/micr.1920150309
Publication status	Published - 1994
Externally published	Yes

Keywords

Animals
Cold Temperature
Ischemia
Male
Muscles
Rats
Rats, Inbred Strains
Surgical Flaps
Time Factors

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Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats
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title = "Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats: A macroscopic and morphological study",

abstract = "The purpose of this study was to define the cold (0 degree C) ischaemic tolerance (CIT) of the latissimus dorsi free flap in inbred rats. Flaps were isolated, stored at 0 degree C for periods of time ranging from 0 to 120 hr and then revascularised and reperfused for 14 days. Thereafter the CIT was assessed macroscopically, and by light microscopy. Electron microscopy was performed in flaps after 0 and 24 hr of reperfusion following a cold ischaemic interval ranging from 0 to 120 hr. From this preliminary study, it was concluded that the CIT was 48-72 hr.",

keywords = "Animals, Cold Temperature, Ischemia, Male, Muscles, Rats, Rats, Inbred Strains, Surgical Flaps, Time Factors",

author = "Werker, {P M} and M Kon and Green, {C J} and Fryer, {P R} and Franken, {R J} and Overgoor, {M L}",

year = "1994",

doi = "10.1002/micr.1920150309",

language = "English",

volume = "15",

pages = "187--192",

journal = "Microsurgery",

issn = "0738-1085",

number = "3",

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T1 - Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats

T2 - A macroscopic and morphological study

AU - Werker, P M

AU - Kon, M

AU - Green, C J

AU - Fryer, P R

AU - Franken, R J

AU - Overgoor, M L

PY - 1994

Y1 - 1994

N2 - The purpose of this study was to define the cold (0 degree C) ischaemic tolerance (CIT) of the latissimus dorsi free flap in inbred rats. Flaps were isolated, stored at 0 degree C for periods of time ranging from 0 to 120 hr and then revascularised and reperfused for 14 days. Thereafter the CIT was assessed macroscopically, and by light microscopy. Electron microscopy was performed in flaps after 0 and 24 hr of reperfusion following a cold ischaemic interval ranging from 0 to 120 hr. From this preliminary study, it was concluded that the CIT was 48-72 hr.

AB - The purpose of this study was to define the cold (0 degree C) ischaemic tolerance (CIT) of the latissimus dorsi free flap in inbred rats. Flaps were isolated, stored at 0 degree C for periods of time ranging from 0 to 120 hr and then revascularised and reperfused for 14 days. Thereafter the CIT was assessed macroscopically, and by light microscopy. Electron microscopy was performed in flaps after 0 and 24 hr of reperfusion following a cold ischaemic interval ranging from 0 to 120 hr. From this preliminary study, it was concluded that the CIT was 48-72 hr.

KW - Animals

KW - Cold Temperature

KW - Ischemia

KW - Male

KW - Muscles

KW - Rats

KW - Rats, Inbred Strains

KW - Surgical Flaps

KW - Time Factors

U2 - 10.1002/micr.1920150309

DO - 10.1002/micr.1920150309

M3 - Article

C2 - 8015424

SN - 0738-1085

VL - 15

SP - 187

EP - 192

JO - Microsurgery

JF - Microsurgery

IS - 3

ER -

Cold (0°C) ischaemic tolerance of latissimus dorsi free flaps in rats: A macroscopic and morphological study

Abstract

Keywords

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