Collagen distribution in the human vitreoretinal interface

Theodorus L. Ponsioen*, Marja J. A. van Luyn, Roelofje J. van der Worp, Jan C. van Meurs, Johanna M. M. Hooymans, Leonoor I. Los

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

PURPOSE. To evaluate the presence of collagen types I to VII, IX, XI, and XVIII at the posterior pole, the equator and the preequatorial area in human donor eyes, since collagens are important macromolecules that contribute to vitreoretinal adhesion at the vitreoretinal interface.

METHODS. Freshly isolated human retinectomy samples from the equator were used for reverse transcription-polymerase chain reaction to detect mRNA of the above-mentioned collagens. In addition, human donor eyes and equatorial retinectomy samples were embedded in paraffin, stained with antibodies against the collagens and evaluated by light microscopy (LM).

RESULTS. Retinectomy samples expressed mRNA of all tested collagen types. By LM, vitreous cortex was positive for collagen types II, V, IX, and XI. In all three regions within the donor eyes and in the retinectomy samples, the internal limiting membrane (ILM) showed types IV, VI, and XVIII; the retinal vasculature was positive for types I to VI and XVIII in most specimens; and the retinal layers showed condensed spots of type VII. In addition, type VII increased in density and in distribution over the retinal layers toward the posterior pole.

CONCLUSIONS. Staining patterns of collagen types I to V, IX, XI, and XVIII confirmed previous observations. Important new findings include the presence of type VI in the ILM and type VII in several layers of the retina. Both collagens can anchor matrix components, and type VI could be involved in vitreoretinal attachment. Furthermore, the presence of collagen mRNA in human retinectomy samples may be an indication of postnatal collagen production by retinal cells.

Original languageEnglish
Pages (from-to)4089-4095
Number of pages7
JournalInvestigative ophthalmology & visual science
Volume49
Issue number9
DOIs
Publication statusPublished - Sep-2008

Keywords

  • EXTRACELLULAR-MATRIX COMPONENTS
  • POSTERIOR VITREOUS DETACHMENT
  • RETINAL-PIGMENT EPITHELIUM
  • HUMAN OCULAR-TISSUES
  • VI COLLAGEN
  • SUPRAMOLECULAR ORGANIZATION
  • CHOROID TRANSLOCATION
  • MACULAR DEGENERATION
  • ANCHORING FIBRILS
  • BASEMENT-MEMBRANE

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