Bacteria are unicellular organisms present all over the world. Normally, each bacterium increases its length size before splitting into two identical cells. Fundamental to these life-cycle steps are two different, yet related cellular processes named cell wall synthesis and cell division. The cell wall synthesis process is important to ensure that the cell wall layer, responsible for setting the cell boundary and shape, is properly synthesized and organized. The synthesis of new cell wall material requires different proteins that work together to produce peptidoglycan, the main cell wall component. Proper cell wall synthesis allows for the extension of the cell wall layer while the cell increases its length and is equally necessary to add new cell wall material at the division site. Cell division occurs at about the midcell and is a combined approach of different proteins that ultimately divide one bacterium into two daughter-cells. Both cell wall synthesis and cell division are highly important and thus have been extensively studied in the past decades; however some fine details are still unknown. In this thesis, different approaches are used to unravel some complexities associated with cell wall synthesis and cell division. Different aspects are investigated, such as the internal organization of the membrane, the chaperone activity of YidC and the antibacterial effect of alkyl gallates. This study underlines the intricate structure behind the bacterial cell wall synthesis and cell division.
|Translated title of the contribution||Kom buitenspelen: Verkenningen van bacteriële celwandsynthese en celdeling|
|Qualification||Doctor of Philosophy|
|Place of Publication||[Groningen]|
|Publication status||Published - 2017|