Comparative Study of Photoswitchable Zinc-Finger Domain and AT-Hook Motif for Light-Controlled Peptide-DNA Binding

Gosia M. Murawska, Claudia Poloni, Nadja A. Simeth, Wiktor Szymanski*, Ben L. Feringa

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)
76 Downloads (Pure)

Abstract

DNA-peptide interactions are involved in key life processes, including DNA recognition, replication, transcription, repair, organization, and modification. Development of tools that can influence DNA-peptide binding non-invasively with high spatiotemporal precision could aid in determining its role in cells and tissues. Here, the design, synthesis, and study of photocontrolled tools for sequence-specific small peptide-DNA major and minor groove interactions are reported, shedding light on DNA binding by transcriptionally active peptides. In particular, photoswitchable moieties were implemented in the peptide backbone or turn region. In each case, DNA binding was affected by photochemical isomerization, as determined in fluorescent displacement assays on model DNA strands, which provides promising tools for DNA modulation.

Original languageEnglish
Pages (from-to)4965-4973
Number of pages9
JournalChemistry
Volume25
Issue number19
DOIs
Publication statusPublished - 1-Apr-2019

Keywords

  • AT-hook
  • DNA binding
  • peptides
  • photoswitching
  • zinc finger
  • ETHIDIUM-BROMIDE
  • REVERSIBLE PHOTOCONTROL
  • CONVENIENT ACTINOMETER
  • PROTEIN CONFORMATION
  • CHROMOSOMAL-PROTEINS
  • PHOTO-CONTROL
  • IN-VIVO
  • AZOBENZENE
  • TRANSCRIPTION
  • RECOGNITION

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