Comparison of reversed-phase column materials for high-performance liquid chromatography of proteins

Ruurd van der Zee *, Thea Hoekzema, Sytske Welling - Wester, Gjalt W. Welling

*Corresponding author for this work

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    Nine reversed-phase materials with various bonded phases from different suppliers were studied for the separation of hydrophilic proteins with two solvent systems. Protein retention, resolution and recovery were not correlated with the nature of the hydrocarbonaceous ligand. Peak volumes increased with molecular weight, which led to broad, irregular peaks for the larger proteins on some columns.
    Four columns that performed equally well were selected for the purification of hydrophobic Sendai virus membrane proteins. In this case, more distinct differences were found between columns. Recovery of the membrane proteins strongly depended on the combination of column and solvent systems
    Original languageEnglish
    Pages (from-to)283-289
    Number of pages7
    JournalJournal of Chromatography A
    Issue number2
    Publication statusPublished - 24-Oct-1986

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