Comparison of three methods for competitive binding of monoclonal antibodies - The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin

FG Perton, JH Dijkema, T Smilda, BE vanUffelen, JJ Beintema

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    Abstract

    The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods, A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity, Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.

    Original languageEnglish
    Pages (from-to)117-125
    Number of pages9
    JournalJournal of Immunological Methods
    Volume190
    Issue number1
    Publication statusPublished - 28-Mar-1996

    Keywords

    • competitive binding
    • hemocyanin
    • Panulirus interruptus
    • antigenic region
    • epitope
    • 3-DIMENSIONAL STRUCTURE
    • PROTEIN
    • CHROMATOGRAPHY
    • EPITOPES
    • COMPLEX
    • SUBUNIT

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