The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods, A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity, Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.
|Number of pages||9|
|Journal||Journal of Immunological Methods|
|Publication status||Published - 28-Mar-1996|
- competitive binding
- Panulirus interruptus
- antigenic region
- 3-DIMENSIONAL STRUCTURE