Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library

Jurre Hageman, Harm H. Kampinga*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    99 Citations (Scopus)

    Abstract

    In this manuscript, we describe the generation of a gene library for the expression of HSP110/HSPH, HSP70/HSPA and HSP40/DNAJ members. First, the heat shock protein (HSP) genes were collected from the gene databases and the gene families were analyzed for expression patterns, heat inducibility, subcellular localization, and protein homology using several bioinformatics approaches. These results can be used as a working draft model until data are confirmed by experimental approaches. In addition, we describe the generation of a HSPA/DNAJ overexpression library and tested the effect of different fusion tags on HSPA and DNAJ members using different techniques for measuring chaperone activity. These results show that we have cloned a high-quality heat shock protein expression library containing most members from the HSPH, HSPA, DNAJA and DNAJB families which will be useful for the chaperone community to unravel the function of the highly diverse family of human molecular chaperones.

    Original languageEnglish
    Pages (from-to)1-21
    Number of pages21
    JournalCell stress & chaperones
    Volume14
    Issue number1
    DOIs
    Publication statusPublished - Jan-2009

    Keywords

    • HspH
    • HspA
    • DnaJ
    • Hsp110
    • Hsp70
    • Hsp40
    • Human chaperones
    • Bio-informatics
    • PROTEIN SUBCELLULAR-LOCALIZATION
    • MAMMALIAN-CELLS
    • INCLUSION FORMATION
    • CHAPERONE ACTIVITY
    • IN-VIVO
    • HSP70
    • GENE
    • PREDICTION
    • GENOME
    • INFORMATION

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