Computational approaches for RNA structure ensemble deconvolution from structure probing data

Sharon Aviran*, Danny Incarnato*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)
7 Downloads (Pure)

Abstract

RNA structure probing experiments have emerged over the last decade as a straightforward way to determine the structure of RNA molecules in a number of different contexts. Although powerful, the ability of RNA to dynamically interconvert between, and to simultaneously populate, alternative structural configurations, poses a nontrivial challenge to the interpretation of data derived from these experiments. Recent efforts aimed at developing computational methods for the reconstruction of coexisting alternative RNA conformations from structure probing data are paving the way to the study of RNA structure ensembles, even in the context of living cells. In this review, we critically discuss these methods, their limitations and possible future improvements.

Original languageEnglish
Article number167635
Number of pages23
JournalJournal of Molecular Biology
Volume434
Issue number18
Early online date17-May-2022
DOIs
Publication statusPublished - 30-Sep-2022

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