CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS

MHJ RUITERS; EA VANSPRONSEN; OH SKJELDAL; P STROMME; HR SCHOLTE; H PZYREMBEL; GPA SMIT; W RUITENBEEK; E AGSTERIBBE

CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS

MHJ RUITERS, EA VANSPRONSEN, OH SKJELDAL, P STROMME, HR SCHOLTE, H PZYREMBEL, GPA SMIT, W RUITENBEEK, E AGSTERIBBE

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Abstract

This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethyl-aminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

Original language	English
Pages (from-to)	45-48
Number of pages	4
Journal	Journal of Inherited Metabolic Disease
Volume	14
Issue number	1
Publication status	Published - 1991

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DASPMI

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title = "CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS",

abstract = "This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethyl-aminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.",

keywords = "DASPMI",

author = "MHJ RUITERS and EA VANSPRONSEN and OH SKJELDAL and P STROMME and HR SCHOLTE and H PZYREMBEL and GPA SMIT and W RUITENBEEK and E AGSTERIBBE",

year = "1991",

language = "English",

volume = "14",

pages = "45--48",

journal = "Journal of Inherited Metabolic Disease",

issn = "0141-8955",

publisher = "Springer",

number = "1",

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TY - JOUR

T1 - CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS

AU - RUITERS, MHJ

AU - VANSPRONSEN, EA

AU - SKJELDAL, OH

AU - STROMME, P

AU - SCHOLTE, HR

AU - PZYREMBEL, H

AU - SMIT, GPA

AU - RUITENBEEK, W

AU - AGSTERIBBE, E

PY - 1991

Y1 - 1991

N2 - This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethyl-aminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

AB - This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethyl-aminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

KW - DASPMI

M3 - Article

SN - 0141-8955

VL - 14

SP - 45

EP - 48

JO - Journal of Inherited Metabolic Disease

JF - Journal of Inherited Metabolic Disease

IS - 1

ER -

CONFOCAL SCANNING LASER MICROSCOPY OF MITOCHONDRIA - A POSSIBLE TOOL IN THE DIAGNOSIS OF MITOCHONDRIAL DISORDERS

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