Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis

Josephus van der Vossen; Jan Kok; Gerhardus Venema

Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis

Josephus van der Vossen, Jan Kok, Gerhardus Venema

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

73 Citations (Scopus)

170 Downloads (Pure)

Abstract

Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

Original language	English
Pages (from-to)	540-542
Number of pages	3
Journal	Applied and environmental microbiology
Volume	50
Issue number	2
Publication status	Published - Aug-1985

Access to Document

1985ApplEnvirMicrobiolvdVossenFinal publisher's version, 535 KB

Handle.net

Persistent link

Cite this

@article{c60bed8442c848db959dfa2113ab6141,

title = "Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis",

abstract = "Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.",

author = "\{van der Vossen\}, Josephus and Jan Kok and Gerhardus Venema",

note = "Relation: http://www.rug.nl/gbb/ date\_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1985",

month = aug,

language = "English",

volume = "50",

pages = "540--542",

journal = "Applied and environmental microbiology",

issn = "0099-2240",

publisher = "AMER SOC MICROBIOLOGY",

number = "2",

}

Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis. / van der Vossen, Josephus; Kok, Jan; Venema, Gerhardus.
In: Applied and environmental microbiology, Vol. 50, No. 2, 08.1985, p. 540-542.

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

TY - JOUR

T1 - Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis

AU - van der Vossen, Josephus

AU - Kok, Jan

AU - Venema, Gerhardus

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1985/8

Y1 - 1985/8

N2 - Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

AB - Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

M3 - Comment/Letter to the editor

SN - 0099-2240

VL - 50

SP - 540

EP - 542

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

IS - 2

ER -

Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus subtilis and Streptococcus lactis

Abstract

Access to Document

Handle.net

Fingerprint

Cite this