Contraction-induced muscle fiber damage is increased in soleus muscle of streptozotocin-diabetic rats and is associated with elevated expression of brain-derived neurotrophic factor mRNA in muscle fibers and activated satellite cells

S Copray*, R Liem, N Brouwer, P Greenhaff, F Habens, P Fernyhough

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

The expression of brain-derived neurotrophic factor (BDNF) is elevated in the soleus muscle of streptozotocin-diabetic rats. To determine whether this diabetes-induced elevation was associated with or enhanced by muscle activity we have induced high-intensity muscle contraction by electrically stimulating the sciatic nerve. In 6-week diabetic rats, intense contraction of the soleus muscle resulted in a two- to four-fold elevation of BDNF mRNA and increased plasma levels of creatine kinase that were associated with severe focal muscle fiber damage and concomitant satellite cell activation. Focal muscle fiber damage and concomitant satellite cell activation were also observed in the soleus muscle of nonstimulated diabetic rats, but to a much lesser extent. No effects of muscle contraction, i.e., experimentally induced or during normal daily activity, on muscle fiber structure or BDNF mRNA expression were seen in diabetic extensor digitorum longus (EDL) muscle. Using a nonradioactive in situ hybridization technique for electron microscopy, the elevated expression of BDNF mRNA in the diabetic soleus muscle was localized within muscle fibers as web as activated satellite cells. This study shows that diabetic soleus muscle, in contrast to diabetic EDL and to soleus and EDL muscle of normal animals, is highly susceptible to contraction-induced damage. Intense contraction and the associated muscle fiber damage in the diabetic soleus muscle result in an upregulation of BDNF mRNA in muscle fibers and activated satellite cells, which may be involved in the restoration and/or maintenance of nerve/muscle integrity. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)597-608
Number of pages12
JournalExperimental Neurology
Volume161
Issue number2
Publication statusPublished - Feb-2000

Keywords

  • neuropathy
  • neurotrophic factor
  • neurotrophin-3
  • electron microscopy
  • in situ hybridization
  • demyelinization
  • MESSENGER-RNA LEVELS
  • SKELETAL-MUSCLE
  • DIAMINOBENZIDINE REACTION
  • CREATINE-KINASE
  • GROWTH-FACTORS
  • MOTOR-NEURONS
  • NEUROPATHY
  • EXERCISE
  • NERVE
  • RECEPTORS

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