Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato: Plants with a different susceptibility to non-freezing temperature

Maciej Garstka*, Jan Henk Venema, Izabela Rumak, Katarzyna Gieczewska, Malgorzata Rosiak, Joanna Koziol-Lipinska, Borys Kierdaszuk, Wim J. Vredenberg, Agnieszka Mostowska

*Corresponding author for this work

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    Abstract

    The effect of dark-chilling and subsequent photoactivation on chloroplast structure and arrangements of chlorophyll-protein complexes in thylakoid membranes was studied in chilling-tolerant (CT) pea and in chilling-sensitive (CS) tomato. Dark-chilling did not influence chlorophyll content and Chl a/b ratio in thylakoids of both species. A decline of Chl a fluorescence intensity and an increase of the ratio of fluorescence intensities of PSI and PSII at 120 K was observed after dark-chilling in thylakoids isolated from tomato, but not from pea leaves. Chilling of pea leaves induced an increase of the relative contribution of LHCII and PSII fluorescence. A substantial decrease of the LHCII/PSII fluorescence accompanied by an increase of that from LHCI/PSI was observed in thylakoids from chilled tomato leaves; both were attenuated by photoactivation. Chlorophyll fluorescence of bright grana discs in chloroplasts from dark-chilled leaves, detected by confocal laser scanning microscopy, was more condensed in pea but significantly dispersed in tomato, compared with control samples. The chloroplast images from transmission-electron microscopy revealed that dark-chilling induced an increase of the degree of grana stacking only in pea chloroplasts. Analyses of O-J-D-I-P fluorescence induction curves in leaves of CS tomato before and after recovery from chilling indicate changes in electron transport rates at acceptor- and donor side of PS II and an increase in antenna size. In CT pea leaves these effects were absent, except for a small but irreversible effect on PSII activity and antenna size. Thus, the differences in chloroplast structure between CS and CT plants, induced by dark-chilling are a consequence of different thylakoid supercomplexes rearrangements.

    Original languageEnglish
    Pages (from-to)1165-1181
    Number of pages17
    JournalPlanta
    Volume226
    Issue number5
    DOIs
    Publication statusPublished - Oct-2007

    Keywords

    • chloroplast and thylakoid membrane structure
    • chilling-sensitive
    • chilling tolerant
    • chlorophyll-protein complexes
    • cation-induced thylakoid stacking
    • O-J-D-I-P chlorophyll fluorescence
    • confocal laser scanning microscopy
    • dark-chilling stress
    • pea
    • tomato
    • PHOTOSYNTHETIC ELECTRON-TRANSPORT
    • HILL REACTION ACTIVITY
    • FATTY-ACID LEVELS
    • THYLAKOID MEMBRANES
    • PHOTOSYSTEM-I
    • CUCUMBER LEAVES
    • ARABIDOPSIS-THALIANA
    • SUBSEQUENT RECOVERY
    • SOYBEAN GENOTYPES
    • COLD-ACCLIMATION

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