Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site

Dirk-Jan Scheffers; Carine Robichon; Gert Jan Haan; Tanneke den Blaauwen; Gregory Koningstein; Edwin van Bloois; Jon Beckwith; Joen Luirink

doi:10.1128/JB.00723-07

Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site

Dirk-Jan Scheffers, Carine Robichon, Gert Jan Haan, Tanneke den Blaauwen, Gregory Koningstein, Edwin van Bloois, Jon Beckwith, Joen Luirink

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

355 Downloads (Pure)

Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

Original language	English
Pages (from-to)	7273-7280
Number of pages	8
Journal	Journal of Bacteriology
Volume	189
Issue number	20
DOIs	https://doi.org/10.1128/JB.00723-07
Publication status	Published - 2007

Access to Document

10.1128/JB.00723-07

2007JBacteriolScheffers.pdfFinal publisher's version, 325 KB

Handle.net

Persistent link

Cite this

@article{fdfa386297e84ed4b4ca6dbe92290221,

title = "Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site",

abstract = "The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.",

author = "Dirk-Jan Scheffers and Carine Robichon and Haan, {Gert Jan} and Blaauwen, {Tanneke den} and Gregory Koningstein and Bloois, {Edwin van} and Jon Beckwith and Joen Luirink",

note = "Relation: http://www.rug.nl/fmns-research/gbb/index Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)",

year = "2007",

doi = "10.1128/JB.00723-07",

language = "English",

volume = "189",

pages = "7273--7280",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

number = "20",

}

TY - JOUR

T1 - Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site

AU - Scheffers, Dirk-Jan

AU - Robichon, Carine

AU - Haan, Gert Jan

AU - Blaauwen, Tanneke den

AU - Koningstein, Gregory

AU - Bloois, Edwin van

AU - Beckwith, Jon

AU - Luirink, Joen

N1 - Relation: http://www.rug.nl/fmns-research/gbb/index Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)

PY - 2007

Y1 - 2007

N2 - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

AB - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.

U2 - 10.1128/JB.00723-07

DO - 10.1128/JB.00723-07

M3 - Article

SN - 0021-9193

VL - 189

SP - 7273

EP - 7280

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 20

ER -

Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site

Abstract

Access to Document

Handle.net

Fingerprint

Cite this