Abstract
An important limitation in cell therapy for the regeneration of tissue is the initial lack of oxygen. After implantation of large 3D cell-seeded structures, cells die rather than contribute to tissue regenerating. Here we've tested oxygen-releasing materials to improve cell survival and growth after implantation. Calcium peroxide (CaO2) in a polymer matrix was used as source of oxygen. Two polymers were tested in order to slow down and extend the period of oxygen release, poly(D,L-lactic acid) and poly(lactic-co-glycolic acid). Compared to CaO2 particles, both releasing systems showed an initially higher and shorter oxygen release. Human mesenchymal stromal cells cultured on casted films of these oxygen-releasing composites required catalase to proliferate, indicating the production of cytotoxic hydrogen peroxide as intermediate. Poly(D, L-lactic acid) and poly(lactic-co-glycolic acid) are less suited for slowly oxygen-releasing materials. Catalase was able to reduce the cytotoxic effect of H2O2.
Original language | English |
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Article number | 207 |
Number of pages | 4 |
Journal | Journal of Materials Science : Materials in Medicine |
Volume | 26 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul-2015 |
Keywords
- DELIVERY-SYSTEMS
- BIOMATERIALS
- DEGRADATION
- KINETICS
- CELLS
- ACID)