Control of oxygen release from peroxides using polymers

Hilde Steg, Arina T. Buizer, Willem Woudstra, Albert G. Veldhuizen, Sjoerd K. Bulstra, Dirk W. Grijpma, Roel Kuijer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

An important limitation in cell therapy for the regeneration of tissue is the initial lack of oxygen. After implantation of large 3D cell-seeded structures, cells die rather than contribute to tissue regenerating. Here we've tested oxygen-releasing materials to improve cell survival and growth after implantation. Calcium peroxide (CaO2) in a polymer matrix was used as source of oxygen. Two polymers were tested in order to slow down and extend the period of oxygen release, poly(D,L-lactic acid) and poly(lactic-co-glycolic acid). Compared to CaO2 particles, both releasing systems showed an initially higher and shorter oxygen release. Human mesenchymal stromal cells cultured on casted films of these oxygen-releasing composites required catalase to proliferate, indicating the production of cytotoxic hydrogen peroxide as intermediate. Poly(D, L-lactic acid) and poly(lactic-co-glycolic acid) are less suited for slowly oxygen-releasing materials. Catalase was able to reduce the cytotoxic effect of H2O2.

Original languageEnglish
Article number207
Number of pages4
JournalJournal of Materials Science : Materials in Medicine
Volume26
Issue number7
DOIs
Publication statusPublished - Jul-2015

Keywords

  • DELIVERY-SYSTEMS
  • BIOMATERIALS
  • DEGRADATION
  • KINETICS
  • CELLS
  • ACID)

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