Controlled Translocation of Individual DNA Molecules through Protein Nanopores with Engineered Molecular Brakes

Marcela Rincon-Restrepo, Elina Milthallova, Hagan Bayley, Giovanni Maglia

Research output: Contribution to journalArticleAcademicpeer-review

101 Citations (Scopus)


Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the alpha-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site directed mutagenesis. Although the residual ionic current during DNA translocation is insufficient for direct base identification, we propose that the engineered pores might be used to slow down DNA in hybrid systems, for example, in combination with solid-state nanopores.
Original languageEnglish
Pages (from-to)746-750
Number of pages5
JournalNano Letters
Issue number2
Publication statusPublished - Feb-2011
Externally publishedYes


  • alpha-hemolysin
  • DNA sequencing
  • protein engineering
  • DNA translocation

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