COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins

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Abstract

COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis.

Original languageEnglish
Pages (from-to)508-511
Number of pages4
JournalThorax
Volume76
Issue number5
Early online date3-Dec-2020
DOIs
Publication statusPublished - May-2021

Keywords

  • COPD mechanisms
  • COPD pathology
  • CELLULAR SENESCENCE
  • GENE-EXPRESSION
  • LUNG
  • PARAQUAT

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