Correlated light and electron microscopy: ultrastructure lights up!

Pascal de Boer, Jacob P. Hoogenboom, Ben N. G. Giepmans*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

227 Citations (Scopus)

Abstract

Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light microscope. A spectrum of dyes and probes now enable the localization of molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure has been revealed. Bridging these two modalities, correlated light microscopy and EM (CLEM) opens new avenues. Studies of protein dynamics with fluorescent proteins (FPs), which leave the investigator 'in the dark' concerning cellular context, can be followed by EM examination. Rare events can be preselected at the light microscopy level before EM analysis. Ongoing development-including of dedicated probes, integrated microscopes, large-scale and three-dimensional EM and super-resolution fluorescence microscopy-now paves the way for broad CLEM implementation in biology.

Original languageEnglish
Pages (from-to)503-513
Number of pages11
JournalNature Methods
Volume12
Issue number6
DOIs
Publication statusPublished - Jun-2015

Keywords

  • FLUORESCENCE MICROSCOPY
  • SUPERRESOLUTION FLUORESCENCE
  • HIGH-RESOLUTION
  • CRYOELECTRON TOMOGRAPHY
  • EUKARYOTIC CELLS
  • SPATIAL PRECISION
  • CRYO-FLUORESCENCE
  • INTEGRATED LIGHT
  • FIDUCIAL MARKERS
  • MAMMALIAN-CELLS

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