Correlated Light Microscopy and Electron Microscopy

Klaas A. Sjollema*, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan, Ben N. G. Giepmans

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

33 Citations (Scopus)

Abstract

Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Recent developments in fluorescence light microscopy (FLM) allow protein imaging in living cells and at the near molecular level.

However, fluorescence microscopy only reveals selected biomolecules or organelles but not the (ultra)structural context, as can be examined by electron microscopy (EM). LM and EM of the same cells, so-called correlative (or correlated) light and electron microscopy (CLEM), allow examining rare or dynamic events first by LM, and subsequently by EM. Here, we review progress in CLEM, with focus on matching the areas between different microscopic modalities. Moreover, we introduce a method that includes a virtual overlay and automated large-scale imaging, allowing to switch between most microscopes. Ongoing developments will revolutionize and standardize CLEM in the near future, which thus holds great promise to become a routine technique in cell biology.

Original languageEnglish
Title of host publicationCORRELATIVE LIGHT AND ELECTRON MICROSCOPY
EditorsT MullerReichert, P Verkade
Place of PublicationSAN DIEGO
PublisherAcademic Press
Pages157-173
Number of pages17
ISBN (Print)978-0-12-416026-2
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Cell Biology
PublisherELSEVIER ACADEMIC PRESS INC
Volume111
ISSN (Print)0091-679X

Keywords

  • SUPERRESOLUTION FLUORESCENCE MICROSCOPY
  • ULTRATHIN CRYOSECTIONS
  • CRYOELECTRON TOMOGRAPHY
  • CELL MONOLAYERS
  • QUANTUM DOTS
  • IN-SITU
  • TISSUES
  • TOOL
  • IMMUNOCYTOCHEMISTRY
  • PHOTOOXIDATION

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