Corticosterone metabolism by chicken follicle cells does not affect ovarian reproductive hormone synthesis in vitro

Sophie Rettenbacher*, Rie Henriksen, Ton G. Groothuis, Michael Lepschy

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
553 Downloads (Pure)

Abstract

Glucocorticoids affect reproductive hormone production in many species. In chickens, elevated plasma corticosterone down-regulates testosterone and progesterone concentrations in plasma, but also in egg yolk. This suppression could be mediated via the hypothalamic-pituitary system but also via local inhibition of gonadal activity by glucocorticoids. As the latter has not been tested in birds yet, we tested if corticosterone directly inhibits ovarian steroid synthesis under in vitro conditions. We hypothesized that degradation of corticosterone by follicular cells impairs their ability to synthesize reproductive hormones due to either inhibition Of enzymes or competition for common co-factors. Therefore, we first established whether follicles degrade corticosterone. Follicular tissue was harvested from freshly euthanized laying hens and incubated with radiolabelled corticosterone. Radioactive metabolites were visualized and quantified by autoradiography. Follicles converted corticosterone in a time-dependent manner into metabolites with a higher polarity than corticosterone. The predominant metabolite co-eluted with 20 beta-dihydrocorticosterone. Other chicken tissues mostly formed the same metabolite when incubated with corticosterone. In a second experiment, follicles were incubated with either progesterone or dehydroepiandrosterone. Corticosterone was added in increasing dosages up to 1000 ng per ml medium. Corticosterone did not inhibit the conversion of progesterone and dehydroepiandrosterone into a number of different metabolites, including 17 alpha-hydroxyprogesterone, androstenedione and testosterone. In conclusion, avian tissues degrade corticosterone mostly to 20 beta-dihydrocorticosterone and even high corticosterone dosages do not affect follicular hormone production under in vitro conditions. (C) 2013 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume184
Issue number4
DOIs
Publication statusPublished - 1-Apr-2013

Keywords

  • Stress
  • Reproduction
  • HPA-axis
  • HPG-axis
  • Poultry
  • beta-Imaging
  • RAT LEYDIG-CELLS
  • MESSENGER-RNA EXPRESSION
  • FOWL GALLUS-DOMESTICUS
  • ADULT MALE-RATS
  • 11-BETA-HYDROXYSTEROID DEHYDROGENASE
  • IMMOBILIZATION STRESS
  • GRANULOSA-CELLS
  • TESTICULAR STEROIDOGENESIS
  • TESTOSTERONE BIOSYNTHESIS
  • GONADOTROPIN-SECRETION

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