Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia

BI Kapanadze; VM Brodyanskii; AB Semov; AV Baranova; GE Sulimova; SS Aitova; IG Udina; SN Ptitsyna; LE Salnikova; OS Chudinov; TE Borbiev; VV Kashuba; R Gizatullin; [No Value] Zabarovska; ER Zabarovsky; LI Fedorova; AV Zelenin; O Rasool; D Grander; S Einhorn; W vanEverdink; Anke van den Berg; C Buys; M Corcoran; RM Chapman; NK Yankovsky

Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia

BI Kapanadze, VM Brodyanskii, AB Semov, AV Baranova, GE Sulimova, SS Aitova, IG Udina, SN Ptitsyna, LE Salnikova, OS Chudinov, TE Borbiev, VV Kashuba, R Gizatullin, [No Value] Zabarovska, ER Zabarovsky, LI Fedorova, AV Zelenin, O Rasool, D Grander, S EinhornW vanEverdink, Anke van den Berg, C Buys, M Corcoran, RM Chapman, NK Yankovsky

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We constructed a fine physical map of human chromosome 13q14 region between D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. This interval had been shown to be in the center of the genome region frequently lost in a human blood malignancy known as B-cell chronic lymphocytic leukemia (BCLL), Mapping of the genome region is a step in searching for a putative tumor suppressor gene for BCLL. A chr13-specific cosmid library (LA13NC01) was screened with four YAC and seven WI-STS markers belonging to the above 13q14 interval, yielding a cosmid subset of more than 400 clones representing the region. Cosmids between D13S1168 and D13S25 loci were arranged in contigs. Seven different clones were found in cDNA libraries by hybridization screening with YAC ICRF66c1 and two cosmids covering the D13S319 locus, which is the central point for BCLL-associated deletions, The cDNA clones were mapped against the contigous cosmids.

Original language	English
Pages (from-to)	431-434
Number of pages	4
Journal	Molecular biology
Volume	31
Issue number	3
Publication status	Published - 1997

Keywords

human chromosome 13
13q14 region
cosmid map
cDNA map
chronic lymphocytic leukemia
D13S25 LOCUS
LIBRARIES
DELETION
GENE
CONSTRUCTION
BREAKPOINT

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Kapanadze, BI., Brodyanskii, VM., Semov, AB., Baranova, AV., Sulimova, GE., Aitova, SS., Udina, IG., Ptitsyna, SN., Salnikova, LE., Chudinov, OS., Borbiev, TE., Kashuba, VV., Gizatullin, R., Zabarovska, N. V., Zabarovsky, ER., Fedorova, LI., Zelenin, AV., Rasool, O., Grander, D., ... Yankovsky, NK. (1997). Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia. Molecular biology, 31(3), 431-434.

@article{f37a85cb25a447dc9f085bf9f41691b3,

title = "Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia",

abstract = "We constructed a fine physical map of human chromosome 13q14 region between D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. This interval had been shown to be in the center of the genome region frequently lost in a human blood malignancy known as B-cell chronic lymphocytic leukemia (BCLL), Mapping of the genome region is a step in searching for a putative tumor suppressor gene for BCLL. A chr13-specific cosmid library (LA13NC01) was screened with four YAC and seven WI-STS markers belonging to the above 13q14 interval, yielding a cosmid subset of more than 400 clones representing the region. Cosmids between D13S1168 and D13S25 loci were arranged in contigs. Seven different clones were found in cDNA libraries by hybridization screening with YAC ICRF66c1 and two cosmids covering the D13S319 locus, which is the central point for BCLL-associated deletions, The cDNA clones were mapped against the contigous cosmids.",

keywords = "human chromosome 13, 13q14 region, cosmid map, cDNA map, chronic lymphocytic leukemia, D13S25 LOCUS, LIBRARIES, DELETION, GENE, CONSTRUCTION, BREAKPOINT",

author = "BI Kapanadze and VM Brodyanskii and AB Semov and AV Baranova and GE Sulimova and SS Aitova and IG Udina and SN Ptitsyna and LE Salnikova and OS Chudinov and TE Borbiev and VV Kashuba and R Gizatullin and Zabarovska, {[No Value]} and ER Zabarovsky and LI Fedorova and AV Zelenin and O Rasool and D Grander and S Einhorn and W vanEverdink and {van den Berg}, Anke and C Buys and M Corcoran and RM Chapman and NK Yankovsky",

year = "1997",

language = "English",

volume = "31",

pages = "431--434",

journal = "Molecular biology",

issn = "0026-8933",

number = "3",

}

Kapanadze, BI, Brodyanskii, VM, Semov, AB, Baranova, AV, Sulimova, GE, Aitova, SS, Udina, IG, Ptitsyna, SN, Salnikova, LE, Chudinov, OS, Borbiev, TE, Kashuba, VV, Gizatullin, R, Zabarovska, NV, Zabarovsky, ER, Fedorova, LI, Zelenin, AV, Rasool, O, Grander, D, Einhorn, S, vanEverdink, W, van den Berg, A, Buys, C, Corcoran, M, Chapman, RM & Yankovsky, NK 1997, 'Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia', Molecular biology, vol. 31, no. 3, pp. 431-434.

TY - JOUR

T1 - Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia

AU - Kapanadze, BI

AU - Brodyanskii, VM

AU - Semov, AB

AU - Baranova, AV

AU - Sulimova, GE

AU - Aitova, SS

AU - Udina, IG

AU - Ptitsyna, SN

AU - Salnikova, LE

AU - Chudinov, OS

AU - Borbiev, TE

AU - Kashuba, VV

AU - Gizatullin, R

AU - Zabarovska, [No Value]

AU - Zabarovsky, ER

AU - Fedorova, LI

AU - Zelenin, AV

AU - Rasool, O

AU - Grander, D

AU - Einhorn, S

AU - vanEverdink, W

AU - van den Berg, Anke

AU - Buys, C

AU - Corcoran, M

AU - Chapman, RM

AU - Yankovsky, NK

PY - 1997

Y1 - 1997

N2 - We constructed a fine physical map of human chromosome 13q14 region between D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. This interval had been shown to be in the center of the genome region frequently lost in a human blood malignancy known as B-cell chronic lymphocytic leukemia (BCLL), Mapping of the genome region is a step in searching for a putative tumor suppressor gene for BCLL. A chr13-specific cosmid library (LA13NC01) was screened with four YAC and seven WI-STS markers belonging to the above 13q14 interval, yielding a cosmid subset of more than 400 clones representing the region. Cosmids between D13S1168 and D13S25 loci were arranged in contigs. Seven different clones were found in cDNA libraries by hybridization screening with YAC ICRF66c1 and two cosmids covering the D13S319 locus, which is the central point for BCLL-associated deletions, The cDNA clones were mapped against the contigous cosmids.

AB - We constructed a fine physical map of human chromosome 13q14 region between D13S1168 and D13S25 loci consisting of cosmid and cDNA clones. This interval had been shown to be in the center of the genome region frequently lost in a human blood malignancy known as B-cell chronic lymphocytic leukemia (BCLL), Mapping of the genome region is a step in searching for a putative tumor suppressor gene for BCLL. A chr13-specific cosmid library (LA13NC01) was screened with four YAC and seven WI-STS markers belonging to the above 13q14 interval, yielding a cosmid subset of more than 400 clones representing the region. Cosmids between D13S1168 and D13S25 loci were arranged in contigs. Seven different clones were found in cDNA libraries by hybridization screening with YAC ICRF66c1 and two cosmids covering the D13S319 locus, which is the central point for BCLL-associated deletions, The cDNA clones were mapped against the contigous cosmids.

KW - human chromosome 13

KW - 13q14 region

KW - cosmid map

KW - cDNA map

KW - chronic lymphocytic leukemia

KW - D13S25 LOCUS

KW - LIBRARIES

KW - DELETION

KW - GENE

KW - CONSTRUCTION

KW - BREAKPOINT

M3 - Article

SN - 0026-8933

VL - 31

SP - 431

EP - 434

JO - Molecular biology

JF - Molecular biology

IS - 3

ER -

Cosmid contig and cDNA map of the human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia

Abstract

Keywords

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