Covering all bases in HIV research: Unveiling a hidden world of viral evolution

Bram Vrancken*, Sébastian Lequime, Kristof Theys, Philippe Lemey

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

24 Citations (Scopus)


The advent of next-generation sequencing technologies has greatly impacted genomic research by providing a means for increasing the amount of sequence information in a cost effective fashion. Among the major next-generation sequencing technologies, the Roche 454 platform has been widely adopted in HIV research. We discuss a broad range of applications of the 454 pyrosequencing platform in the HIV field, with a particular emphasis on antiretroviral therapy and virus-host interaction-related research. We also highlight some of the bioinformatics challenges, as well as advantages and potential limitations of this "deep" sequencing tool, and hint at future research applications.

Original languageEnglish
Pages (from-to)89-102
Number of pages14
JournalAids reviews
Issue number2
Publication statusPublished - 24-Jun-2010
Externally publishedYes


  • Anti-HIV Agents/therapeutic use
  • Evolution, Molecular
  • HIV/classification
  • HIV Infections/drug therapy
  • Humans
  • RNA, Viral/genetics
  • Sequence Analysis, DNA

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