Creating peroxidase-oxidase fusion enzymes as toolbox for cascade reactions

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Abstract

In this work we prepared a set of bifunctional oxidase-peroxidases by fusing four distinct oxidases to a peroxidase. While such fusion enzymes have not been observed in nature, they could be expressed and purified with good yields. Characterization revealed that the artificial enzymes retained the capability to bind the two required cofactors and were catalytically active as oxidase and peroxidase. The peroxidase fusions of alditol oxidase and chitooligosaccharide oxidase could be used for selective detection of xylitol and cellobiose with a detection limit in the low µM range. The peroxidase fusions of eugenol oxidase and 5-hydroxymethylfurfural oxidase could be used for dioxygen-driven one-pot two-step cascade reactions to convert vanillyl alcohol into divanillin and eugenol into lignin oligomers, respectively. The designed oxidase-peroxidase fusions represent attractive biocatalysts that allow efficient biocatalytic cascade oxidations that only require molecular oxygen as oxidant.

Original languageEnglish
Article numbercbic.201700478
Pages (from-to)2226-2230
Number of pages5
JournalChemBioChem
Volume18
Early online date8-Sep-2017
DOIs
Publication statusPublished - Nov-2017

Keywords

  • enzyme fusion
  • oxidase
  • peroxidase
  • toolbox
  • cascade reactions
  • GENERATION
  • COMPLEXES
  • BIOTECHNOLOGICAL APPLICATIONS
  • CHITOOLIGOSACCHARIDE OXIDASE
  • FUSARIUM-GRAMINEARUM
  • DIVANILLIN
  • BIFUNCTIONAL ENZYME
  • LIGNIN DEGRADATION
  • DISCOVERY
  • MUTAGENESIS

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