Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger

K.-H. Schröter; A. Arkema; H.C.M. Kester; J. Visser; B.W. Dijkstra

doi:10.1006/jmbi.1994.1660

Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger

K.-H. Schröter, A. Arkema, H.C.M. Kester, J. Visser, B.W. Dijkstra

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

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Abstract

The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ray analysis.

Original language	English
Pages (from-to)	351-352
Number of pages	2
Journal	Journal of Molecular Biology
Volume	243
Issue number	2
DOIs	https://doi.org/10.1006/jmbi.1994.1660
Publication status	Published - 21-Oct-1994

Keywords

ENDO-POLYGALACTURONASE II
PROTEIN CRYSTALLIZATION
X-RAY DIFFRACTION

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title = "Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger",

abstract = "The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ray analysis.",

keywords = "ENDO-POLYGALACTURONASE II, PROTEIN CRYSTALLIZATION, X-RAY DIFFRACTION",

author = "K.-H. Schr{\"o}ter and A. Arkema and H.C.M. Kester and J. Visser and B.W. Dijkstra",

note = "Relation: http://www.rug.nl/gbb/ date\_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1994",

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day = "21",

doi = "10.1006/jmbi.1994.1660",

language = "English",

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journal = "Journal of Molecular Biology",

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T1 - Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger

AU - Schröter, K.-H.

AU - Arkema, A.

AU - Kester, H.C.M.

AU - Visser, J.

AU - Dijkstra, B.W.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1994/10/21

Y1 - 1994/10/21

N2 - The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ray analysis.

AB - The endo-polygalacturonase II from Aspergillus niger has been crystallized from an ammonium sulfate solution by the hanging drop method. The crystals belong to the monoclinic space group P2(1), with cell dimensions a = 60.6 Angstrom, b = 152.6 Angstrom, c = 74.0 Angstrom and beta = 91.2 degrees with four molecules per asymmetric unit. The crystals diffract to at least 2.8 Angstrom resolution and are suitable for X-ray analysis.

KW - ENDO-POLYGALACTURONASE II

KW - PROTEIN CRYSTALLIZATION

KW - X-RAY DIFFRACTION

U2 - 10.1006/jmbi.1994.1660

DO - 10.1006/jmbi.1994.1660

M3 - Comment/Letter to the editor

SN - 0022-2836

VL - 243

SP - 351

EP - 352

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 2

ER -

Crystallization and Preliminary Crystallographic Characterization of Endo-polygalacturonase II from Aspergillus niger

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