Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

Robert van Montfort, Tjaard Pijning, Kornelis Kalk, Gea K. Schuurman-Wolters, Jonathan Reizer, Milton H. Safer Jr., George Robillard, Bauke W. Dijkstra

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

7 Citations (Scopus)

Abstract

Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
Original languageEnglish
Pages (from-to)588-590
Number of pages3
JournalJournal of Molecular Biology
Volume239
Issue number4
DOIs
Publication statusPublished - 17-Jun-1994

Keywords

  • CRYSTALLIZATION
  • X-RAY
  • CELLOBIOSE TRANSPORT
  • PERMEASE
  • PHOSPHOTRANSFERASE
  • PHOSPHOENOLPYRUVATE
  • PHOSPHORYLATION
  • TRANSPORT
  • PROTEINS
  • BACTERIA
  • DETECTOR

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