Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

Robert van Montfort; Tjaard Pijning; Kornelis Kalk; Gea K. Schuurman-Wolters; Jonathan Reizer; Milton H. Safer Jr.; George Robillard; Bauke W. Dijkstra

doi:10.1006/jmbi.1994.1399

Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

Robert van Montfort, Tjaard Pijning, Kornelis Kalk, Gea K. Schuurman-Wolters, Jonathan Reizer, Milton H. Safer Jr., George Robillard, Bauke W. Dijkstra

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

7 Citations (Scopus)

Abstract

Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.

Original language	English
Pages (from-to)	588-590
Number of pages	3
Journal	Journal of Molecular Biology
Volume	239
Issue number	4
DOIs	https://doi.org/10.1006/jmbi.1994.1399
Publication status	Published - 17-Jun-1994

Keywords

CRYSTALLIZATION
X-RAY
CELLOBIOSE TRANSPORT
PERMEASE
PHOSPHOTRANSFERASE
PHOSPHOENOLPYRUVATE
PHOSPHORYLATION
TRANSPORT
PROTEINS
BACTERIA
DETECTOR

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@article{5148d91381ec448e9cb04a082fd340b6,

title = "Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli",

abstract = "Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 {\AA} resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 {\AA}, b = 31.7 {\AA}, c = 60.0 {\AA} and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.",

keywords = "CRYSTALLIZATION, X-RAY, CELLOBIOSE TRANSPORT, PERMEASE, PHOSPHOTRANSFERASE, PHOSPHOENOLPYRUVATE, PHOSPHORYLATION, TRANSPORT, PROTEINS, BACTERIA, DETECTOR",

author = "{van Montfort}, Robert and Tjaard Pijning and Kornelis Kalk and Schuurman-Wolters, {Gea K.} and Jonathan Reizer and {Safer Jr.}, {Milton H.} and George Robillard and Dijkstra, {Bauke W.}",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1994",

month = jun,

day = "17",

doi = "10.1006/jmbi.1994.1399",

language = "English",

volume = "239",

pages = "588--590",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "4",

}

Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli. / van Montfort, Robert; Pijning, Tjaard; Kalk, Kornelis; Schuurman-Wolters, Gea K.; Reizer, Jonathan; Safer Jr., Milton H.; Robillard, George; Dijkstra, Bauke W.

In: Journal of Molecular Biology, Vol. 239, No. 4, 17.06.1994, p. 588-590.

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

TY - JOUR

T1 - Crystallization of Enzyme IIB of the Cellobiose-specific Phosphotransferase System of Escherichia coli

AU - van Montfort, Robert

AU - Pijning, Tjaard

AU - Kalk, Kornelis

AU - Schuurman-Wolters, Gea K.

AU - Reizer, Jonathan

AU - Safer Jr., Milton H.

AU - Robillard, George

AU - Dijkstra, Bauke W.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1994/6/17

Y1 - 1994/6/17

N2 - Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.

AB - Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.

KW - CRYSTALLIZATION

KW - X-RAY

KW - CELLOBIOSE TRANSPORT

KW - PERMEASE

KW - PHOSPHOTRANSFERASE

KW - PHOSPHOENOLPYRUVATE

KW - PHOSPHORYLATION

KW - TRANSPORT

KW - PROTEINS

KW - BACTERIA

KW - DETECTOR

U2 - 10.1006/jmbi.1994.1399

DO - 10.1006/jmbi.1994.1399

M3 - Comment/Letter to the editor

VL - 239

SP - 588

EP - 590

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -