Abstract
Crystals of enzyme IIB of the cellobiose-specific phosphotransferase system have been obtained from 15% polyethylene glycol 4000 using both streak-seeding and macroseeding techniques at 4°. Crystals were grown with the hanging drop method of vapour diffusion. Addition of 2-propanol and benzamidine/HCl proved essential to obtain single crystals suitable for X-ray analysis. The crystals diffract to 1.8 Å resolution and have the monoclinic space group P21, with cell dimensions a = 53.6 Å, b = 31.7 Å, c = 60.0 Å and β = 101.7°. From a self-rotation function it seems likely that there are two molecules in the asymmetric unit related by a non-crystallographic 2-fold axis.
Original language | English |
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Pages (from-to) | 588-590 |
Number of pages | 3 |
Journal | Journal of Molecular Biology |
Volume | 239 |
Issue number | 4 |
DOIs |
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Publication status | Published - 17-Jun-1994 |
Keywords
- CRYSTALLIZATION
- X-RAY
- CELLOBIOSE TRANSPORT
- PERMEASE
- PHOSPHOTRANSFERASE
- PHOSPHOENOLPYRUVATE
- PHOSPHORYLATION
- TRANSPORT
- PROTEINS
- BACTERIA
- DETECTOR