The A-domain of the mannitol transport protein enzyme IImtl from Escherichia coli (relative molecular mass 16,300) was crystallized, both at room temperature and 4°C, from 40% polyethylene glycol 6000 (pH 8.5 to 9.0) using the hanging-drop method of vapour diffusion. The crystals have the monoclinic space group P21, with unit cell dimensions a = 54.0 Å, b = 67.0 Å, c = 80.9 Å and β = 100.8°. They diffract to 2.6 Å resolution. A self-rotation function and self-Patterson suggest that there are four molecules in the asymmetric unit showing mmm symmetry.
- MANNITOL TRANSPORT
- BACTERIAL PHOSPHOTRANSFERASE SYSTEM