The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically, the invention relates to a maethod of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.
|IPC||C12R 1/ 19 A N|
|Publication status||Published - 24-Oct-1996|