Cytochrome P450 OxyBtei catalyzes the first phenolic coupling step in teicoplanin biosynthesis

Kristina Haslinger, Egle Maximowitsch, Clara Brieke, Alexa Koch, Max J Cryle*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)

Abstract

Bacterial cytochrome P450s form a remarkable clade of the P450 superfamily of oxidative hemoproteins, and are often involved in the biosynthesis of complex natural products. Those in a subgroup known as "Oxy enzymes" play a crucial role in the biosynthesis of glycopeptide antibiotics, including vancomycin and teicoplanin. The Oxy enzymes catalyze crosslinking of aromatic residues in the non-ribosomal antibiotic precursor peptide while it remains bound to the non-ribosomal peptide synthetase (NRPS); this crosslinking secures the three-dimensional structure of the glycopeptide, crucial for antibiotic activity. We have characterized OxyBtei , the first of the Oxy enzymes in teicoplanin biosynthesis. Our results reveal that OxyBtei possesses a structure similar to those of other Oxy proteins and is active in crosslinking NRPS-bound peptide substrates. However, OxyBtei displays a significantly altered activity spectrum against peptide substrates compared to its well-studied vancomycin homologue.

Original languageEnglish
Pages (from-to)2719-28
Number of pages10
JournalChemBioChem
Volume15
Issue number18
DOIs
Publication statusPublished - 15-Dec-2014
Externally publishedYes

Keywords

  • Anti-Bacterial Agents/chemistry
  • Bacterial Proteins/chemistry
  • Biocatalysis
  • Cytochrome P-450 Enzyme System/chemistry
  • Micromonosporaceae/chemistry
  • Models, Molecular
  • Phenols/chemistry
  • Teicoplanin/chemistry

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