Cytogenetic analysis of cryopreserved bone marrow cells

AG Bosga-Bouwer; E Vellenga; H Zorgdrager; E van den Berg

Cytogenetic analysis of cryopreserved bone marrow cells

AG Bosga-Bouwer^*
, E Vellenga
, H Zorgdrager
, E van den Berg

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells. (C) 2001 Elsevier Science Inc. All rights reserved.

Original language	English
Pages (from-to)	165-168
Number of pages	4
Journal	Cancer Genetics and Cytogenetics
Volume	124
Issue number	2
Publication status	Published - 15-Jan-2001

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title = "Cytogenetic analysis of cryopreserved bone marrow cells",

abstract = "We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells. (C) 2001 Elsevier Science Inc. All rights reserved.",

author = "AG Bosga-Bouwer and E Vellenga and H Zorgdrager and \{van den Berg\}, E",

year = "2001",

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language = "English",

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T1 - Cytogenetic analysis of cryopreserved bone marrow cells

AU - Bosga-Bouwer, AG

AU - Vellenga, E

AU - Zorgdrager, H

AU - van den Berg, E

PY - 2001/1/15

Y1 - 2001/1/15

N2 - We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells. (C) 2001 Elsevier Science Inc. All rights reserved.

AB - We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells. (C) 2001 Elsevier Science Inc. All rights reserved.

M3 - Article

SN - 0165-4608

VL - 124

SP - 165

EP - 168

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

IS - 2

ER -

Cytogenetic analysis of cryopreserved bone marrow cells

Abstract

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