Cytokine profiles in bronchoalveolar lavage fluid and blood in HIV-seropositive patients with Pneumocystis carinii pneumonia

RM Perenboom*, RW Sauerwein, P Beckers, ACHW VanSchijndel, RP VanSteenwijk, JCC Borleffs, R VanLeusen, JWM VanderMeer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Concentrations and ex vivo production of interleukin 1 beta (IL-1), tumour necrosis alpha (TNF), interleukin 6 (IL-6), interleukin-1 receptor antagonist (IL-1RA) and TNF soluble receptors (sTNF-receptors, P55 and P75) were measured in bronchoalveolar lavage (BAL) fluid and blood in 23 HIV-seropositive (HIV+) patients with Pneumocystis carinii pneumonia (PCP) and compared with Values found in healthy HIV-seronegative (HIV-) controls and asymptomatic HIV+ subjects. Concentrations of the proinflammatory cytokine IL-1 beta were increased in BAL fluid of HIV+ patients with PCP (184 +/- 47 pg mL(-1)) compared with undetectable levels in healthy control subjects (P = 0.0001). In plasma of these patients higher concentrations of the anti-inflammatory cytokine IL-1RA were found during acute PCP than after recovery (2.1 +/- 0.7 vs. 0.5 +/- 0.2 ngmL(-1), P = 0.01). No correlations could be found between cytokine concentrations and clinicaI severity of the infection. Corticosteroid treatment did not influence cytokine concentrations in BAL or blood, nor did it suppress the production in alveolar cells. In whole-blood cultures, however, lipopolysaccharide (LPS)-stimulated production was significantly suppressed for IL-1 (1.3 vs. 5.5 ng mL(-1) P = 0.009) and for IL-6 (0.6 vs. 2.5 ng mL(-1), P = 0.01). The overall data show that in HIV+ patients with PCP (similar to what we had found previously in HIV-patients with PCP) proinflammatory cytokines are more prominently present in BAL, whereas anti-inflammatory reaction is predominant in the circulation.

Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalEuropean Journal of Clinical Investigation
Volume27
Issue number4
DOIs
Publication statusPublished - Apr-1997
Externally publishedYes
Event3rd International-Cytokine-Society Conference - HARROGATE, United Kingdom
Duration: 12-Sept-199515-Sept-1995

Keywords

  • cytokine
  • HIV
  • Pneumocystis carinii pneumonia
  • tumour necrosis factor alpha
  • soluble tumour necrosis factor receptor
  • interleukin 1
  • interleukin 1 receptor antagonist
  • interleukin 6
  • TUMOR-NECROSIS-FACTOR
  • FACTOR-ALPHA
  • ALVEOLAR MACROPHAGES
  • PROINFLAMMATORY CYTOKINES
  • HOST-RESISTANCE
  • INFECTION
  • INVITRO
  • AIDS
  • RECEPTORS
  • CELLS

Fingerprint

Dive into the research topics of 'Cytokine profiles in bronchoalveolar lavage fluid and blood in HIV-seropositive patients with Pneumocystis carinii pneumonia'. Together they form a unique fingerprint.

Cite this