Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity

C. M. Boomsma*, A. Kavelaars, M. J. C. Eijkemans, K. Amarouchi, G. Teklenburg, D. Gutknecht, B. J. C. M. Fauser, C. J. Heijnen, N. S. Macklon

*Corresponding author for this work

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Abstract

Investigation of human embryo implantation requires a non-disruptive means of studying the endometrium during the window of implantation. This stud), describes a novel approach of cytokine profiling in endometrial secretions. Endometrial secretions aspirated prior to embryo transfer from 210 women undergoing IVF or intracytoplasmic sperm injection were analysed by a multiplex immunoassay. Tell mediators [interleukin (IL)-1 beta, IL-6, IL-12, IL-18, tumour necrosis factor-alpha, macrophage migration inhibitory factor, eotaxin, monocyte chemotactic protein-1, interferon-gamma inducible protein-10, vascular endothelial, growth factor] were detectable in 90-100% of the samples. Heparin-binding epidermal growth factor, IL-5, IL-17, IL-10, Dickkopf homologue-1 and IL-15 were detected in 23-76%, whereas interferon-gamma was not detectable in any of the samples. To assess possible contamination of samples, cervical mucus was also aspirated for comparative analysis in 22 women. The endometrial cytokine profile differed significantly from cervical mucus. Pregnancy rates of the study participants who underwent endometrial secretion aspiration were compared with 210 controls matched for important prognostic variables no significant differences were found. In conclusion, cytokine profiling in endometrial secretion offers all objective, non-disruptive means of analysing the in-vivo milieu encountered by the embryo and offers a new and potentially valuable approach to studying the endometrial factor in human embryo implantation.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalReproductive Biomedicine Online
Volume18
Issue number1
Publication statusPublished - Jan-2009

Keywords

  • cytokines
  • endometrial receptivity
  • endometrial secretion
  • IVF
  • EPIDERMAL-GROWTH-FACTOR
  • IN-VITRO FERTILIZATION
  • MIGRATION INHIBITORY FACTOR
  • GENE-EXPRESSION PROFILES
  • NATURAL-KILLER-CELLS
  • UTERINE RECEPTIVITY
  • EMBRYO-TRANSFER
  • IMPLANTATION FAILURE
  • OVARIAN STIMULATION
  • RECEPTOR ANTAGONIST

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