Cytolethal distending toxin in Escherichia coli O157:H7: spectrum of conservation, structure, and endothelial toxicity

Alexander W Friedrich; Shan Lu; Martina Bielaszewska; Rita Prager; Phillip Bruns; Jian-Guo Xu; Helmut Tschäpe; Helge Karch

doi:10.1128/JCM.44.5.1844-1846.2006

Cytolethal distending toxin in Escherichia coli O157:H7: spectrum of conservation, structure, and endothelial toxicity

Alexander W Friedrich, Shan Lu, Martina Bielaszewska, Rita Prager, Phillip Bruns, Jian-Guo Xu, Helmut Tschäpe, Helge Karch

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Abstract

We identified the cytolethal distending toxin V (CDT-V) gene cluster in 19 (4.9%) of 391 enterohemorrhagic Escherichia coli O157:H7. cdt-V+ strains belonged to five phage types (PTs) and were most frequent within PTs 14 and 34. CDT-V was expressed in all but two cdt-V+ strains and was lethal to cultured endothelial cells. Subtyping schemes should include cdt-V as a marker to differentiate E. coli O157:H7 even within the same phage type.

Original language	English
Pages (from-to)	1844-6
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1128/JCM.44.5.1844-1846.2006
Publication status	Published - May-2006
Externally published	Yes

Keywords

Alleles
Bacterial Toxins
Base Sequence
Cell Line
Conserved Sequence
DNA, Bacterial
Diarrhea
Endothelium, Vascular
Escherichia coli Infections
Escherichia coli O157
Evolution, Molecular
Genes, Bacterial
Hemolytic-Uremic Syndrome
Humans
Multigene Family
Virulence

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title = "Cytolethal distending toxin in Escherichia coli O157:H7: spectrum of conservation, structure, and endothelial toxicity",

abstract = "We identified the cytolethal distending toxin V (CDT-V) gene cluster in 19 (4.9%) of 391 enterohemorrhagic Escherichia coli O157:H7. cdt-V+ strains belonged to five phage types (PTs) and were most frequent within PTs 14 and 34. CDT-V was expressed in all but two cdt-V+ strains and was lethal to cultured endothelial cells. Subtyping schemes should include cdt-V as a marker to differentiate E. coli O157:H7 even within the same phage type.",

keywords = "Alleles, Bacterial Toxins, Base Sequence, Cell Line, Conserved Sequence, DNA, Bacterial, Diarrhea, Endothelium, Vascular, Escherichia coli Infections, Escherichia coli O157, Evolution, Molecular, Genes, Bacterial, Hemolytic-Uremic Syndrome, Humans, Multigene Family, Virulence",

author = "Friedrich, {Alexander W} and Shan Lu and Martina Bielaszewska and Rita Prager and Phillip Bruns and Jian-Guo Xu and Helmut Tsch{\"a}pe and Helge Karch",

year = "2006",

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doi = "10.1128/JCM.44.5.1844-1846.2006",

language = "English",

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T1 - Cytolethal distending toxin in Escherichia coli O157:H7

T2 - spectrum of conservation, structure, and endothelial toxicity

AU - Friedrich, Alexander W

AU - Lu, Shan

AU - Bielaszewska, Martina

AU - Prager, Rita

AU - Bruns, Phillip

AU - Xu, Jian-Guo

AU - Tschäpe, Helmut

AU - Karch, Helge

PY - 2006/5

Y1 - 2006/5

N2 - We identified the cytolethal distending toxin V (CDT-V) gene cluster in 19 (4.9%) of 391 enterohemorrhagic Escherichia coli O157:H7. cdt-V+ strains belonged to five phage types (PTs) and were most frequent within PTs 14 and 34. CDT-V was expressed in all but two cdt-V+ strains and was lethal to cultured endothelial cells. Subtyping schemes should include cdt-V as a marker to differentiate E. coli O157:H7 even within the same phage type.

AB - We identified the cytolethal distending toxin V (CDT-V) gene cluster in 19 (4.9%) of 391 enterohemorrhagic Escherichia coli O157:H7. cdt-V+ strains belonged to five phage types (PTs) and were most frequent within PTs 14 and 34. CDT-V was expressed in all but two cdt-V+ strains and was lethal to cultured endothelial cells. Subtyping schemes should include cdt-V as a marker to differentiate E. coli O157:H7 even within the same phage type.

KW - Alleles

KW - Bacterial Toxins

KW - Base Sequence

KW - Cell Line

KW - Conserved Sequence

KW - DNA, Bacterial

KW - Diarrhea

KW - Endothelium, Vascular

KW - Escherichia coli Infections

KW - Escherichia coli O157

KW - Evolution, Molecular

KW - Genes, Bacterial

KW - Hemolytic-Uremic Syndrome

KW - Humans

KW - Multigene Family

KW - Virulence

U2 - 10.1128/JCM.44.5.1844-1846.2006

DO - 10.1128/JCM.44.5.1844-1846.2006

M3 - Article

C2 - 16672418

SN - 0095-1137

VL - 44

SP - 1844

EP - 1846

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Cytolethal distending toxin in Escherichia coli O157:H7: spectrum of conservation, structure, and endothelial toxicity

Abstract

Keywords

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