Cytosol-mimetic chemistry: Kinetics of the trypsin-catalyzed hydrolysis of p-nitrophenyl acetate upon addition of polyethylene glycol and N-tert-butyl acetoacetamide

N. Asaad; J.B.F.N. Engberts

doi:10.1021/ja034298f

Cytosol-mimetic chemistry: Kinetics of the trypsin-catalyzed hydrolysis of p-nitrophenyl acetate upon addition of polyethylene glycol and N-tert-butyl acetoacetamide

N. Asaad, J.B.F.N. Engberts

Faculty of Science and Engineering

Research output: Contribution to journal › Article › Academic › peer-review

42 Citations (Scopus)

484 Downloads (Pure)

Original language	English
Pages (from-to)	6874-6875
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	23
DOIs	https://doi.org/10.1021/ja034298f
Publication status	Published - 11-Jun-2003

Keywords

ORGANIC-SOLVENTS
WATER
PROTEINS

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title = "Cytosol-mimetic chemistry: Kinetics of the trypsin-catalyzed hydrolysis of p-nitrophenyl acetate upon addition of polyethylene glycol and N-tert-butyl acetoacetamide",

keywords = "ORGANIC-SOLVENTS, WATER, PROTEINS",

author = "N. Asaad and J.B.F.N. Engberts",

note = "Copyright (C) 2010 American Chemical Society (ACS). All Rights Reserved.; Section Code: 7-3; CODEN: JACSAT; CAS Registry Numbers: 830-03-5 (p-Nitrophenyl Acetate); 9002-07-7 (Trypsin) Role: BSU (Biological study, unclassified), BIOL (Biological study) (large concn. of polyethylene glycol and N-tert-Bu acetoacetamide solutes has modest effect on kinetics of trypsin-catalyzed hydrolysis of p-nitrophenyl acetate); 25322-68-3 (Polyethylene Glycol); 42222-06-0 (N-tert-Butyl acetoacetamide) Role: NUU (Other use, unclassified), USES (Uses) (large concn. of polyethylene glycol and N-tert-Bu acetoacetamide solutes has modest effect on kinetics of trypsin-catalyzed hydrolysis of p-nitrophenyl acetate)",

year = "2003",

month = jun,

day = "11",

doi = "10.1021/ja034298f",

language = "English",

volume = "125",

pages = "6874--6875",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "AMER CHEMICAL SOC",

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AU - Asaad, N.

AU - Engberts, J.B.F.N.

N1 - Copyright (C) 2010 American Chemical Society (ACS). All Rights Reserved.; Section Code: 7-3; CODEN: JACSAT; CAS Registry Numbers: 830-03-5 (p-Nitrophenyl Acetate); 9002-07-7 (Trypsin) Role: BSU (Biological study, unclassified), BIOL (Biological study) (large concn. of polyethylene glycol and N-tert-Bu acetoacetamide solutes has modest effect on kinetics of trypsin-catalyzed hydrolysis of p-nitrophenyl acetate); 25322-68-3 (Polyethylene Glycol); 42222-06-0 (N-tert-Butyl acetoacetamide) Role: NUU (Other use, unclassified), USES (Uses) (large concn. of polyethylene glycol and N-tert-Bu acetoacetamide solutes has modest effect on kinetics of trypsin-catalyzed hydrolysis of p-nitrophenyl acetate)

PY - 2003/6/11

Y1 - 2003/6/11

KW - ORGANIC-SOLVENTS

KW - WATER

KW - PROTEINS

U2 - 10.1021/ja034298f

DO - 10.1021/ja034298f

M3 - Article

SN - 0002-7863

VL - 125

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EP - 6875

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 23

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