Decidual memory T‐cell subsets and memory T‐cell stimulatory cytokines in early‐ and late‐onset preeclampsia

Tom E C Kieffer, Anne Laskewitz, Annegé Vledder, Sicco A Scherjon, Marijke M Faas, Jelmer R Prins*

*Corresponding author for this work

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Abstract

Problem: Preeclampsia is a major cause of fetal and maternal mortality and morbidity. Disturbed fetal-maternal immune tolerance, and therewith memory T cells, might be involved in its etiology. This study aims to give insight into memory T-cell populations and its associated cytokines in the decidual layers in early-onset preeclampsia (EO-PE) and late-onset preeclampsia (LO-PE). Method of Study: Lymphocytes were isolated from the decidua parietalis and basalis from EO-PE (n = 6), LO-PE (n = 8) and healthy (n = 15) pregnancies. CD4+ and CD8+ central- (CCR7+), effector- (CCR7), tissue resident- (CD103+), and regulatory- (Foxp3+) memory cell (CD45RO+) populations and their activation status (CD69+) were analyzed using flow cytometry. qRT-PCR analysis was performed on decidua parietalis and basalis biopsies to detect mRNA expression of interferon-gamma, interleukin-1B, IL2, IL6, IL7, IL8, IL10, IL15, and IL23. Results: CD4+ central-memory (CM) cell proportions were lower in the decidua parietalis in LO-PE (P <.0001) and EO-PE (P <.01) compared to healthy pregnancies. CD8+ memory (P <.05) and CD8+ CM (P <.01) cell proportions were also lower in the decidua parietalis in EO-PE compared to healthy pregnancies. This was accompanied by higher IL15 (P <.05) and IL23 (P <.05) and lower IL7 (P <.05) mRNA expression in decidua basalis biopsies from EO-PE compared to healthy pregnancies, analyzed by qPCR. Conclusion: In conclusion, decidual memory T-cell proportions, their activation status, and associated cytokines are altered in preeclampsia and might therefore be involved in fetal-maternal immune tolerance and the pathophysiology of preeclampsia.

Original languageEnglish
Article number13293
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume84
Issue number4
Early online date23-Jun-2020
DOIs
Publication statusPublished - 8-Jul-2020

Keywords

  • decidua
  • early-onset preeclampsia
  • late-onset preeclampsia
  • memory T cell
  • pregnancy
  • HUMAN PLACENTA
  • DIFFERENTIAL DISTRIBUTION
  • IMMUNE-RESPONSE
  • PREGNANCY
  • PHENOTYPE
  • PROMOTES
  • GENERATION
  • EXPRESSION
  • TOLERANCE
  • SURVIVAL

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