Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice

Vincent van Rompaey; Alex Liesenborghs; Karel Goyvaerts; Daan de Herd; Dorien Verdoodt; Marc Lammers; Paul van de Heyning; Olivier Vanderveken; Krystyna Szewczyk; Sofie Thys; Isabel Pintelon; Jean Pierre Timmermans; Jordi Llorens; Peter de Deyn; Debby van Dam

doi:10.5152/B-ENT.2021.21452

Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice

Vincent van Rompaey^*, Alex Liesenborghs, Karel Goyvaerts, Daan de Herd, Dorien Verdoodt, Marc Lammers, Paul van de Heyning, Olivier Vanderveken, Krystyna Szewczyk, Sofie Thys, Isabel Pintelon, Jean Pierre Timmermans, Jordi Llorens, Peter de Deyn, Debby van Dam

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Objective: sensorineural hearing loss (SNHL) and bilateral vestibulopathy (BV) have been associated with cognitive decline and incident dementia. Our aim was to investigate the combined effect of profound SNHL and BV on spatial cognition and hippocampal neurogenesis in adult mice.

Methods: Single oral intake of allylnitrile produces otovestibular failure in less than a week. Behavioral assessment included recording of spontaneous activity, motor activity, spatial cognition, etc. Evaluation of hippocampal neurogenesis was performed 8 weeks after treatment by quantification of neural precursor cells and proliferating cells in the dentate gyrus by staining with doublecortin (Dcx) and Ki67, respectively.

Results: Profound SNHL and BV were confirmed in the allylnitrile-treated mice respectively by means of auditory brainstem response (ABR) and acoustic startle response, and several vestibular tests. Spatial cognitive deficits, i.e. higher latency to target, were observed with the Barnes maze. In the right hemisphere, no statistically significant difference was observed between groups. In the left hemisphere, the difference in mean cell densities of Dcx positive cells was statistically significant when compared to the control group, whereas the difference in mean cell density of Ki67 positive cells did not differ significantly.

Conclusion: Spatial cognitive deficits and decreased immunoreactivity to DCX in the left hippocampus were observed 8 weeks after adult mice acquired profound SNHL and BV.

Original language	English
Pages (from-to)	223-233
Number of pages	11
Journal	B-Ent
Volume	17
Issue number	4
DOIs	https://doi.org/10.5152/B-ENT.2021.21452
Publication status	Published - Oct-2021

Keywords

Acquired deafness
auditory perception
cognition
hair cells
hearing
BRAIN-STEM RESPONSE
BEHAVIORAL ABNORMALITIES
COGNITIVE IMPAIRMENT
SYSTEMIC TOXICITY
CAUSATIVE FACTORS
SPATIAL MEMORY
FOLLOW-UP
ISOFLURANE
ALLYLNITRILE
WALKING

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van Rompaey, V., Liesenborghs, A., Goyvaerts, K., de Herd, D., Verdoodt, D., Lammers, M., van de Heyning, P., Vanderveken, O., Szewczyk, K., Thys, S., Pintelon, I., Timmermans, J. P., Llorens, J., de Deyn, P., & van Dam, D. (2021). Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice. B-Ent, 17(4), 223-233. https://doi.org/10.5152/B-ENT.2021.21452

@article{acc326c07c464f59b40d5c540776108c,

title = "Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice",

abstract = "Objective: sensorineural hearing loss (SNHL) and bilateral vestibulopathy (BV) have been associated with cognitive decline and incident dementia. Our aim was to investigate the combined effect of profound SNHL and BV on spatial cognition and hippocampal neurogenesis in adult mice.Methods: Single oral intake of allylnitrile produces otovestibular failure in less than a week. Behavioral assessment included recording of spontaneous activity, motor activity, spatial cognition, etc. Evaluation of hippocampal neurogenesis was performed 8 weeks after treatment by quantification of neural precursor cells and proliferating cells in the dentate gyrus by staining with doublecortin (Dcx) and Ki67, respectively.Results: Profound SNHL and BV were confirmed in the allylnitrile-treated mice respectively by means of auditory brainstem response (ABR) and acoustic startle response, and several vestibular tests. Spatial cognitive deficits, i.e. higher latency to target, were observed with the Barnes maze. In the right hemisphere, no statistically significant difference was observed between groups. In the left hemisphere, the difference in mean cell densities of Dcx positive cells was statistically significant when compared to the control group, whereas the difference in mean cell density of Ki67 positive cells did not differ significantly.Conclusion: Spatial cognitive deficits and decreased immunoreactivity to DCX in the left hippocampus were observed 8 weeks after adult mice acquired profound SNHL and BV.",

keywords = "Acquired deafness, auditory perception, cognition, hair cells, hearing, BRAIN-STEM RESPONSE, BEHAVIORAL ABNORMALITIES, COGNITIVE IMPAIRMENT, SYSTEMIC TOXICITY, CAUSATIVE FACTORS, SPATIAL MEMORY, FOLLOW-UP, ISOFLURANE, ALLYLNITRILE, WALKING",

author = "{van Rompaey}, Vincent and Alex Liesenborghs and Karel Goyvaerts and {de Herd}, Daan and Dorien Verdoodt and Marc Lammers and {van de Heyning}, Paul and Olivier Vanderveken and Krystyna Szewczyk and Sofie Thys and Isabel Pintelon and Timmermans, {Jean Pierre} and Jordi Llorens and {de Deyn}, Peter and {van Dam}, Debby",

year = "2021",

month = oct,

doi = "10.5152/B-ENT.2021.21452",

language = "English",

volume = "17",

pages = "223--233",

journal = "B-Ent",

issn = "0001-6497",

publisher = "ROYAL BELGIAN SOCIETY EAR, NOSE, THROAT, HEAD & NECK SURGERY",

number = "4",

}

van Rompaey, V, Liesenborghs, A, Goyvaerts, K, de Herd, D, Verdoodt, D, Lammers, M, van de Heyning, P, Vanderveken, O, Szewczyk, K, Thys, S, Pintelon, I, Timmermans, JP, Llorens, J, de Deyn, P & van Dam, D 2021, 'Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice', B-Ent, vol. 17, no. 4, pp. 223-233. https://doi.org/10.5152/B-ENT.2021.21452

TY - JOUR

T1 - Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice

AU - van Rompaey, Vincent

AU - Liesenborghs, Alex

AU - Goyvaerts, Karel

AU - de Herd, Daan

AU - Verdoodt, Dorien

AU - Lammers, Marc

AU - van de Heyning, Paul

AU - Vanderveken, Olivier

AU - Szewczyk, Krystyna

AU - Thys, Sofie

AU - Pintelon, Isabel

AU - Timmermans, Jean Pierre

AU - Llorens, Jordi

AU - de Deyn, Peter

AU - van Dam, Debby

PY - 2021/10

Y1 - 2021/10

N2 - Objective: sensorineural hearing loss (SNHL) and bilateral vestibulopathy (BV) have been associated with cognitive decline and incident dementia. Our aim was to investigate the combined effect of profound SNHL and BV on spatial cognition and hippocampal neurogenesis in adult mice.Methods: Single oral intake of allylnitrile produces otovestibular failure in less than a week. Behavioral assessment included recording of spontaneous activity, motor activity, spatial cognition, etc. Evaluation of hippocampal neurogenesis was performed 8 weeks after treatment by quantification of neural precursor cells and proliferating cells in the dentate gyrus by staining with doublecortin (Dcx) and Ki67, respectively.Results: Profound SNHL and BV were confirmed in the allylnitrile-treated mice respectively by means of auditory brainstem response (ABR) and acoustic startle response, and several vestibular tests. Spatial cognitive deficits, i.e. higher latency to target, were observed with the Barnes maze. In the right hemisphere, no statistically significant difference was observed between groups. In the left hemisphere, the difference in mean cell densities of Dcx positive cells was statistically significant when compared to the control group, whereas the difference in mean cell density of Ki67 positive cells did not differ significantly.Conclusion: Spatial cognitive deficits and decreased immunoreactivity to DCX in the left hippocampus were observed 8 weeks after adult mice acquired profound SNHL and BV.

AB - Objective: sensorineural hearing loss (SNHL) and bilateral vestibulopathy (BV) have been associated with cognitive decline and incident dementia. Our aim was to investigate the combined effect of profound SNHL and BV on spatial cognition and hippocampal neurogenesis in adult mice.Methods: Single oral intake of allylnitrile produces otovestibular failure in less than a week. Behavioral assessment included recording of spontaneous activity, motor activity, spatial cognition, etc. Evaluation of hippocampal neurogenesis was performed 8 weeks after treatment by quantification of neural precursor cells and proliferating cells in the dentate gyrus by staining with doublecortin (Dcx) and Ki67, respectively.Results: Profound SNHL and BV were confirmed in the allylnitrile-treated mice respectively by means of auditory brainstem response (ABR) and acoustic startle response, and several vestibular tests. Spatial cognitive deficits, i.e. higher latency to target, were observed with the Barnes maze. In the right hemisphere, no statistically significant difference was observed between groups. In the left hemisphere, the difference in mean cell densities of Dcx positive cells was statistically significant when compared to the control group, whereas the difference in mean cell density of Ki67 positive cells did not differ significantly.Conclusion: Spatial cognitive deficits and decreased immunoreactivity to DCX in the left hippocampus were observed 8 weeks after adult mice acquired profound SNHL and BV.

KW - Acquired deafness

KW - auditory perception

KW - cognition

KW - hair cells

KW - hearing

KW - BRAIN-STEM RESPONSE

KW - BEHAVIORAL ABNORMALITIES

KW - COGNITIVE IMPAIRMENT

KW - SYSTEMIC TOXICITY

KW - CAUSATIVE FACTORS

KW - SPATIAL MEMORY

KW - FOLLOW-UP

KW - ISOFLURANE

KW - ALLYLNITRILE

KW - WALKING

U2 - 10.5152/B-ENT.2021.21452

DO - 10.5152/B-ENT.2021.21452

M3 - Article

SN - 0001-6497

VL - 17

SP - 223

EP - 233

JO - B-Ent

JF - B-Ent

IS - 4

ER -

Decreased Doublecortin (DCX) immunoreactivity in hippocampus after profound sensorineural hearing loss and vestibular dysfunction in adult mice

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Keywords

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