Design and synthesis of ATP-based nucleotide analogues and profiling of nucleotide-binding proteins

Justina. C. Wolters, Gerard Roelfes, Bert Poolman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Two nucleotide-based probes were designed and synthesized in order to enrich samples for specific classes of proteins by affinity-based protein profiling. We focused on the profiling of adenine nucleotide-binding proteins. Two properties were considered in the design of the probes: the bait needs to bind adenine nucleotide-binding proteins with high affinity and carry a second functional group suitable and easily accessible for coupling to a chromatography resin. For this purpose, we synthesized p-biotinyl amidobenzoic acid-ATP (p-BABA-ATP) and p-biotinyl aminomethylbenzoic acid-ATP (p-BAMBA-ATP). p-BABA-ATP and p-BAMBA-ATP both bind to ATP-binding cassette (ABC) proteins with at least 10-fold higher affinity than ATP. Several ABC transporters could be enriched using p-BABA-ATP or p-BAMBA-ATP.

Original languageEnglish
Pages (from-to)1345-1353
Number of pages9
JournalBIOCONJUGATE CHEMISTRY
Volume22
Issue number7
DOIs
Publication statusPublished - Jul-2011

Keywords

  • ABC TRANSPORTER OPUA
  • ACTIVITY-BASED PROTEOMICS
  • P-GLYCOPROTEIN
  • LACTOCOCCUS-LACTIS
  • SULFOLOBUS-SOLFATARICUS
  • FUNCTIONAL PROTEOMICS
  • ESCHERICHIA-COLI
  • CATALYTIC CYCLE
  • CLICK CHEMISTRY
  • DOMAIN

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