Design and Synthesis of Reagents for Phage Display Screening of Dehalogenases

Roland J. Pieters; Marko Fennema; Richard M. Kellogg; Dick B. Janssen

doi:10.1016/S0960-894X(98)00697-0

Design and Synthesis of Reagents for Phage Display Screening of Dehalogenases

Roland J. Pieters, Marko Fennema, Richard M. Kellogg, Dick B. Janssen

Groningen Biomolecular Sciences and Biotechnology Institute

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane dehalogenase enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	Bioorganic & Medicinal Chemistry Letters
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/S0960-894X(98)00697-0
Publication status	Published - 18-Jan-1999

Keywords

COMBINATORIAL ANTIBODY LIBRARIES
HALOALKANE DEHALOGENASE
CATALYTIC MECHANISM
BETA-LACTAMASE
SELECTION
STREPTAVIDIN
BACTERIOPHAGE
DEGRADATION

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10.1016/S0960-894X(98)00697-0

1999BioorgMedChemLettPieters.pdfFinal publisher's version, 256 KB

Cite this

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title = "Design and Synthesis of Reagents for Phage Display Screening of Dehalogenases",

abstract = "Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane dehalogenase enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.",

keywords = "COMBINATORIAL ANTIBODY LIBRARIES, HALOALKANE DEHALOGENASE, CATALYTIC MECHANISM, BETA-LACTAMASE, SELECTION, STREPTAVIDIN, BACTERIOPHAGE, DEGRADATION",

author = "Pieters, {Roland J.} and Marko Fennema and Kellogg, {Richard M.} and Janssen, {Dick B.}",

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AU - Pieters, Roland J.

AU - Fennema, Marko

AU - Kellogg, Richard M.

AU - Janssen, Dick B.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2009 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

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KW - COMBINATORIAL ANTIBODY LIBRARIES

KW - HALOALKANE DEHALOGENASE

KW - CATALYTIC MECHANISM

KW - BETA-LACTAMASE

KW - SELECTION

KW - STREPTAVIDIN

KW - BACTERIOPHAGE

KW - DEGRADATION

U2 - 10.1016/S0960-894X(98)00697-0

DO - 10.1016/S0960-894X(98)00697-0

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JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

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