Design and Synthesis of Reagents for Phage Display Screening of Dehalogenases

Roland J. Pieters, Marko Fennema, Richard M. Kellogg, Dick B. Janssen

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Abstract

Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane dehalogenase enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.
Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalBioorganic & Medicinal Chemistry Letters
Volume9
Issue number2
DOIs
Publication statusPublished - 18-Jan-1999

Keywords

  • COMBINATORIAL ANTIBODY LIBRARIES
  • HALOALKANE DEHALOGENASE
  • CATALYTIC MECHANISM
  • BETA-LACTAMASE
  • SELECTION
  • STREPTAVIDIN
  • BACTERIOPHAGE
  • DEGRADATION

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