Destruction of Tissue, Cells and Organelles in Type 1 Diabetic Rats Presented at Macromolecular Resolution

Raimond B. G. Ravelli, Ruby D. Kalicharan, M. Cristina Avramut, Klaas A. Sjollema, Joachim W. Pronk, Freark Dijk, Abraham J. Koster, Jeroen T. J. Visser, Frank G. A. Faas, Ben N. G. Giepmans*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Finding alternatives for insulin therapy and making advances in etiology of type 1 diabetes benefits from a full structural and functional insight into Islets of Langerhans. Electron microscopy (EM) can visualize Islet morphology at the highest possible resolution, however, conventional EM only provides biased snapshots and lacks context. We developed and employed large scale EM and compiled a resource of complete cross sections of rat Islets during immuno-destruction to provide unbiased structural insight of thousands of cells at macromolecular resolution. The resource includes six datasets, totalling 25.000 micrographs, annotated for cellular and ultrastructural changes during autoimmune diabetes. Granulocytes are attracted to the endocrine tissue, followed by extravasation of a pleiotrophy of leukocytes. Subcellullar changes in beta cells include endoplasmic reticulum stress, insulin degranulation and glycogen accumulation. Rare findings include erythrocyte extravasation and nuclear actin-like fibers. While we focus on a rat model of autoimmune diabetes, our approach is general applicable.

Original languageEnglish
Article number1804
Number of pages6
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 8-May-2013

Keywords

  • PRONE BB RATS
  • ELECTRON-MICROSCOPY
  • PANCREATIC-ISLETS
  • BETA-CELL
  • LJUNGAN VIRUS
  • INSULITIS
  • INFLAMMATION
  • LOCALIZATION
  • LANGERHANS
  • INFECTION

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