Detection of anti-neutrophil cytoplasmic antibodies (ANCA) by indirect immunofluorescence

J. J.B.C. van Beers, J. Vanderlocht, C. Roozendaal, J. Damoiseaux*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

    13 Citations (Scopus)

    Abstract

    The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.

    Original languageEnglish
    Title of host publicationAutoantibodies
    Subtitle of host publicationMethods and Protocols
    EditorsGunnar Houen
    PublisherHumana Press
    Pages47-62
    Number of pages16
    DOIs
    Publication statusPublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume1901
    ISSN (Print)1064-3745

    Keywords

    • Anti-neutrophil cytoplasmic antibodies
    • Immunofluorescence
    • Inflammatory bowel disease
    • Vasculitis

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