Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing

Giuseppe Fleres; Natacha Couto; Mariette Lokate; Luc W. M. van der Sluis; Christophe Ginevra; Sophie Jarraud; Ruud H. Deurenberg; John W. Rossen; Silvia Garcia-Cobos; Alex W. Friedrich

doi:10.3390/microorganisms6030071

Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing

Giuseppe Fleres, Natacha Couto, Mariette Lokate, Luc W. M. van der Sluis, Christophe Ginevra, Sophie Jarraud, Ruud H. Deurenberg, John W. Rossen, Silvia Garcia-Cobos^*, Alex W. Friedrich

^*Corresponding author for this work

Microbes in Health and Disease (MHD)

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Abstract

This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 x 10(2) CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.

Original language	English
Article number	71
Number of pages	15
Journal	Microorganisms
Volume	6
Issue number	3
DOIs	https://doi.org/10.3390/microorganisms6030071
Publication status	Published - Sept-2018

Keywords

Legionella spp
whole-genome sequencing (WGS)
core-genome multi-locus sequence typing (cgMLST)
whole-genome multi-locus sequence typing (wgMLST)
next-generation sequencing (NGS)
PNEUMOPHILA
ANNOTATION
RESISTANCE
QUALITY

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Fleres, G., Couto, N., Lokate, M., van der Sluis, L. W. M., Ginevra, C., Jarraud, S., Deurenberg, R. H., Rossen, J. W., Garcia-Cobos, S., & Friedrich, A. W. (2018). Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing. Microorganisms, 6(3), Article 71. https://doi.org/10.3390/microorganisms6030071

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title = "Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing",

abstract = "This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 x 10(2) CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.",

keywords = "Legionella spp, whole-genome sequencing (WGS), core-genome multi-locus sequence typing (cgMLST), whole-genome multi-locus sequence typing (wgMLST), next-generation sequencing (NGS), PNEUMOPHILA, ANNOTATION, RESISTANCE, QUALITY",

author = "Giuseppe Fleres and Natacha Couto and Mariette Lokate and {van der Sluis}, {Luc W. M.} and Christophe Ginevra and Sophie Jarraud and Deurenberg, {Ruud H.} and Rossen, {John W.} and Silvia Garcia-Cobos and Friedrich, {Alex W.}",

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AU - Fleres, Giuseppe

AU - Couto, Natacha

AU - Lokate, Mariette

AU - van der Sluis, Luc W. M.

AU - Ginevra, Christophe

AU - Jarraud, Sophie

AU - Deurenberg, Ruud H.

AU - Rossen, John W.

AU - Garcia-Cobos, Silvia

AU - Friedrich, Alex W.

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N2 - This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 x 10(2) CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.

AB - This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 x 10(2) CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.

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KW - core-genome multi-locus sequence typing (cgMLST)

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KW - next-generation sequencing (NGS)

KW - PNEUMOPHILA

KW - ANNOTATION

KW - RESISTANCE

KW - QUALITY

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JF - Microorganisms

IS - 3

M1 - 71

ER -

Detection of Legionella anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing

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